Airlift Bioreactor Manufacturer for Shear-Sensitive Cell & Microbial Culture

We offer bioreactors (fermenters), animal cell bioreactors, bioreactor shakers, biological shakers, bottle rockers, and control systems, and recognize validation services are essential for meeting regulatory and operational requirements of fermenters and bioreactors.

We offer bioreactors (fermenters), animal cell bioreactors, bioreactor shakers, biological shakers, bottle rockers, and control systems, and recognize validation services are essential for meeting regulatory and operational requirements of fermenters and bioreactors.

Bailun Biotechnology is airlift fermenter manufacturer in China. Products cover all kinds of bioreactors in laboratory, pilot scale and industrial production, including fermenter, animal cell bioreactor, plant cell bioreactor, single-use bioreactor, liquid dispensing system and disposable reactor, etc. Can produce bioreactor of 0.1L-1000KL volume and technical services, and provide intelligent and personalized comprehensive solutions for bioreactors process. Bailun has a large number of experienced fermentation process, biochemical equipment and chemical technical engineers, many famous experts and scholars as the company’s technical consultants, Bailun forever pursuits to create value for customers, and adhere to customer-centric is the core value of Bailun.

1.The fermenter tank body is the place that comes into direct contact with the material. The degree of its surface finish and the cleanliness of its internal components are factors that directly affect the contamination. Therefore, the tank body and related equipment must be cleaned in time after each fermentation and before reuse. When cleaning, attention should be paid to the electrical components and electrode interfaces, and water and moisture should not be allowed to enter.

2.PH and DO electrodes should be taken out before cleaning and maintained according to their requirements.

3.The tank can be cleaned together with water intake, air intake, motor stirring and heating. If it cannot be cleaned after multiple water changes, the top cover should be opened and the internal parts of the tank should be brushed with a soft brush. The method is as follows: control the switch and the power switch, remove the top cover electrode, motor and its connection plug. Unplug the air intake hose condenser quick connector and remove the air intake filter upper hose and connector; remove the top cover together with the stirring vertically upward, and place it horizontally on a flat table, pad it well, do not hit the shaft and impeller, and use a neutral detergent to brush the tank body and internal parts.

4.Note: When moving and cleaning the tank cover, only the shaft sleeve or top cover can be grasped, and the stirring shaft, impeller and other easily deformed parts cannot be grasped. While cleaning, the tank and outlet valve and other O-rings should be checked. If deformation, aging, scratches, etc. are found, they should be replaced in time. After cleaning and reinstalling, the surface and tank rack should be wiped dry with a rag. Tighten the six fastening screws of the tank cover, use balanced force and pay attention to the balance of the gap between the tank and the tank seat. Be especially careful when cleaning the glass fermentation tank, and do not collide during disassembly and assembly; when tightening the fastening screws of the tank cover, the force must be balanced and pay attention to the balance of the gap to avoid damaging the glass tank body.

 The stirring system includes the stirring shaft, stirring paddle, mechanical seal. Bearings, couplings and motors, etc.

 Daily maintenance: Regularly check the operation of the stirring shaft, the heating of the motor and the emission of abnormal noise, and regularly check whether the relevant bolts are loose.

 Check the wear of the mechanical seal every year to ensure the normal operation of the mechanical seal and avoid unnecessary contamination.

 If a double-end mechanical seal is used, always pay attention to the changes in the sterile water level. If the liquid level drops, it means that the seal gap is leaking, which should be handled immediately after the fermentation is completed.

 Check the wear of the oil seal and the carbon brush of the motor, as well as the wear of the oil seal and the bearing every year.

The sterilized air system includes air compressor, coarse filter, fine filter, diaphragm valve and air distributor, etc.

In daily maintenance, attention should be paid to the operation of the air compressor. During the fermentation process, the drain valve of the air storage tank should be drained twice a day. Maintenance and care of the filter The sterilization temperature should be controlled below 125℃

 During the sterilization process, attention should be paid to the sterilization temperature of the filter should be controlled below 125℃. Because the filter material will fall off at too high a temperature, the binder will make the filter ineffective.

Replacement of filter element.

 Because the air contains a lot of dust, the micropores of the filter element will gradually become blocked after the filter has been used for a period of time. On the one hand, this will increase the resistance, causing serious lack of air flow and increased air pressure drop, thereby affecting the ventilation volume, and may cause bacterial contamination. At this time, it needs to be replaced.

 Due to the large differences in air quality in various places, there is no accurate service life, which can generally reach more than 6 months. The replacement standard is that the minimum air flow is not less than 1.5 times the volume of the tank. (The pressure difference between the pressure reducer outlet and the tank pressure is not less than 1kg/cm2)

After each fermentation, the air distributor needs to be cleaned. If the properties of the fermentation liquid are similar to water, water can be added to the tank and then compressed air can be passed through for cleaning. If the fermentation liquid is viscous or contains fine particles, it is best to remove the air distributor, unscrew the plug, and rinse with water. At the same time, ensure that the small holes are ventilated smoothly.