{"id":3871,"date":"2024-08-07T08:56:47","date_gmt":"2024-08-07T08:56:47","guid":{"rendered":"https:\/\/fermentorchina.com\/?p=3871"},"modified":"2025-04-17T09:36:22","modified_gmt":"2025-04-17T09:36:22","slug":"suspended-cell-culture-bioreactor-stirred-tank-bioreactor","status":"publish","type":"post","link":"https:\/\/fermentorchina.com\/es\/suspended-cell-culture-bioreactor-stirred-tank-bioreactor\/","title":{"rendered":"Biorreactor de cultivo celular suspendido: Biorreactor de tanque agitado"},"content":{"rendered":"

The stirred tank bioreactor is one of the most common suspended cell culture bioreactors. It keeps the cells in the culture in a suspended state by mechanical stirring and provides sufficient oxygen and nutrients for cell growth.<\/p>\n

\"300L-animal-cell-bioreactor-BLBIO-300SCUC
300L-animal-cell-bioreactor-BLBIO-300SCUC<\/a><\/figcaption><\/figure>\n

The structure of the stirred tank bioreactor is relatively simple and easy to operate and maintain. However, the shear force and bubble impact caused by stirring on the cells may have an adverse effect on cell growth and product yield. Therefore, the stirring speed and gas intake need to be controlled in the stirred tank bioreactor to balance the mixing of the culture and the gas-liquid mass transfer process.<\/p>\n

\"\"En comparaci\u00f3n con los biorreactores de tanque agitado, <\/span>Biorreactores de transporte a\u00e9reo have better performance in the gas-liquid mass transfer process. Airlift bioreactors achieve gas-liquid mass transfer through the rising movement of bubbles, and the contact area between bubbles and liquid phase is greatly increased. <\/span><\/p>\n

In addition, the liquid flow generated by the rising movement of gas in the airlift bioreactor also helps to mix the suspended cells and reduce the differences in the local liquid environment. Therefore, the airlift bioreactor can provide better oxygen and nutrient supply while reducing the shear force on the suspended cells. However, the structure of the airlift bioreactor is relatively complex, and the operation and maintenance are difficult, requiring special gas devices and control systems.<\/span><\/p>\n

In recent years, the application of single use bioreactors has gradually increased, becoming an emerging suspended cell culture bioreactor. Due to its relatively simple structure and operation, single use bioreactors reduce the workload of reactor cleaning and disinfection, can reduce the risk of cross infection, and save production costs.<\/p>\n

\"Bailun
Bailun 300L mammalian cell culture bioreactors BLBIO-300SCUC<\/figcaption><\/figure>\n

Single use bioreactors also have good scalability and are convenient for mass production. However, single use bioreactors may have some problems in gas-liquid mass transfer and control, such as uneven supply of oxygen and nutrients, and difficulty in adjusting pH and temperature. Therefore, when using single use bioreactors, improvements and optimizations need to be made according to specific circumstances.\"\"\"\"<\/p>\n

In order to better meet the needs of suspended cell culture bioreactors, researchers have continuously explored and developed various new reactor designs in practice. For example, flexible wall suspended cell culture bioreactors can provide better isolation of liquid environment and shear force on cells by changing the wall material and structure of the culture chamber.<\/p>\n

The membrane suspended cell culture bioreactor separates the liquid phase and gas phase through the support of the membrane, reducing the impact and shear force of bubbles on cells. These new reactor designs provide new ideas and directions for the development of suspended cell culture bioreactors.\"\"<\/p>\n

En resumen, los biorreactores de cultivo celular suspendido ocupan una posici\u00f3n importante en el campo de la bioingenier\u00eda, y los distintos tipos de reactores difieren en las condiciones de cultivo, los m\u00e9todos de operaci\u00f3n y las estructuras de dise\u00f1o, cada uno con sus propias ventajas. Con el avance continuo de la ciencia y la tecnolog\u00eda, siguen surgiendo diversos dise\u00f1os de reactores nuevos, lo que ofrece m\u00e1s posibilidades para el desarrollo de biorreactores de cultivo celular suspendido. Nuestra investigaci\u00f3n y aplicaci\u00f3n de biorreactores de cultivo celular suspendido seguir\u00e1n profundiz\u00e1ndose, lo que ayudar\u00e1 al desarrollo futuro de productos biofarmac\u00e9uticos y bioprocesos.<\/p>\n

\"glass
Reactores de cultivo de c\u00e9lulas de mam\u00edferos Bailun, biorreactor de vidrio de pared simple<\/figcaption><\/figure>\n

The stirred tank bioreactor is a common suspended cell culture bioreactor, which is widely used in biopharmaceuticals, biofuels and other bioprocesses. It keeps the cells in the culture in a suspended state by mechanical stirring and provides sufficient oxygen and nutrients for cell growth. The basic structure of the stirred tank bioreactor includes a tank body, a stirring device, an air inlet device, a liquid level control device, a temperature control device, etc.<\/p>\n

The tank body of the stirred tank bioreactor is usually made of stainless steel, which has high corrosion resistance and chemical stability. The stirring device of the stirred tank bioreactor is generally composed of a motor and a stirring paddle, and the intensity and speed of stirring can be controlled by adjusting the speed.<\/p>\n

The air inlet device of the stirred tank bioreactor is used to inject gas, usually air or pure oxygen, into the culture medium to provide oxygen for cell growth. The liquid level control device of the stirred tank bioreactor is used to monitor and control the liquid level of the culture medium to maintain the appropriate volume and mixing degree of the culture medium. The temperature control device of the stirred tank bioreactor is used to maintain the temperature of the culture medium within an appropriate range.<\/p>\n

In a stirred tank bioreactor, the suspension state of cells is usually achieved by the turbulent flow generated by the stirring device. The shape and stirring speed of the stirring device have an important influence on the suspension of cells and the mixing effect of the liquid. Generally speaking, in the process of suspended cell culture, a moderate stirring intensity is required to maintain the suspension state of cells, but too high a stirring speed may cause excessive shear force on the cells, which will have an adverse effect on the integrity and biological activity of the cells.<\/p>\n

Therefore, when using a stirred tank bioreactor, it is necessary to determine the appropriate stirring speed according to the specific cell type and culture conditions.<\/p>\n

\"20L-cell-culture
20L-cell-culture bioreactor BLBIO-20GJC mammalian cell culture bioreactor<\/figcaption><\/figure>\n

El suministro de gas tambi\u00e9n es muy importante para el crecimiento celular y la s\u00edntesis de productos. El ox\u00edgeno es un sustrato necesario para la respiraci\u00f3n y el metabolismo celular, mientras que el di\u00f3xido de carbono es uno de los productos. Por lo tanto, los biorreactores de tanque agitado suelen estar equipados con sistemas de suministro de gas adecuados para suministrar el ox\u00edgeno adecuado al medio de cultivo y controlar el contenido de di\u00f3xido de carbono. El control de la tasa de suministro de gas y la velocidad de flujo es muy importante para mantener el equilibrio de gas y la transferencia de masa del medio de cultivo.<\/p>\n

Adem\u00e1s del suministro de ox\u00edgeno y nutrientes, el valor de pH y la temperatura del l\u00edquido tambi\u00e9n son par\u00e1metros importantes que deben controlarse en el cultivo celular. Los biorreactores de tanque agitado pueden estar equipados con dispositivos de control de pH y temperatura adecuados para mantener la estabilidad del medio de cultivo. El control del pH se logra generalmente agregando \u00e1cido o \u00e1lcali, y el control de la temperatura se puede lograr mediante enfriamiento o calentamiento.<\/p>\n

En el cultivo de c\u00e9lulas en suspensi\u00f3n, los biorreactores de tanque agitado tienen las siguientes ventajas y desventajas:<\/p>\n

Ventajas del biorreactor de tanque agitado<\/b><\/strong>:<\/b><\/strong><\/h2>\n
    \n
  1. Mezcla uniforme: el biorreactor de tanque agitado mezcla las c\u00e9lulas, el medio de cultivo y el gas en el medio de cultivo de manera uniforme a trav\u00e9s del dispositivo de agitaci\u00f3n, de modo que las c\u00e9lulas puedan entrar en contacto completo con los nutrientes y el ox\u00edgeno en el medio de cultivo, promover el crecimiento celular y la producci\u00f3n de metabolitos.<\/li>\n
  2. Condiciones de reacci\u00f3n ajustables: Al ajustar la velocidad de agitaci\u00f3n, la temperatura, el valor del pH y otros par\u00e1metros, se pueden controlar las condiciones de reacci\u00f3n en el proceso de cultivo celular para satisfacer los requisitos de crecimiento y expresi\u00f3n de diferentes c\u00e9lulas.<\/li>\n
  3. F\u00e1cil de operar y controlar: El biorreactor de tanque agitado tiene un dise\u00f1o simple y es f\u00e1cil de operar. Puede monitorear y ajustar varios par\u00e1metros del medio de cultivo, como la temperatura, el valor de pH y el ox\u00edgeno disuelto, en tiempo real para garantizar la estabilidad y la capacidad de control del proceso de cultivo.<\/li>\n
  4. Adecuado para producci\u00f3n a gran escala: el biorreactor de tanque agitado puede realizar cultivos de c\u00e9lulas en suspensi\u00f3n a gran escala, lo que es adecuado para la producci\u00f3n industrial y puede mejorar la eficiencia y el rendimiento de la producci\u00f3n.<\/li>\n<\/ol>\n

    Desventajas<\/b><\/strong>\u00a0<\/b><\/strong>del biorreactor de tanque agitado<\/b><\/strong>:<\/b><\/strong><\/h2>\n
      \n
    1. Transferencia desigual de masa gas-l\u00edquido: la agitaci\u00f3n en el biorreactor de tanque agitado provocar\u00e1 una fuerza de corte y la generaci\u00f3n de burbujas en el l\u00edquido, lo que puede provocar una transferencia desigual de masa de ox\u00edgeno, lo que da como resultado un suministro insuficiente de ox\u00edgeno a algunas c\u00e9lulas, lo que afecta el crecimiento celular y la calidad del producto.<\/li>\n
    2. Dificultad en el manejo de l\u00edquidos de alta viscosidad: Para fluidos de cultivo de alta viscosidad, los biorreactores de tanque agitado pueden encontrar problemas de agitaci\u00f3n dif\u00edcil y alto consumo de energ\u00eda.<\/li>\n
    3. Evite la rotura de c\u00e9lulas: para algunas c\u00e9lulas m\u00e1s fr\u00e1giles, la fuerza de agitaci\u00f3n en el biorreactor de tanque agitado puede provocar la rotura o da\u00f1o de las c\u00e9lulas, lo que afecta el crecimiento y el rendimiento celular.<\/li>\n<\/ol>\n

      Los biorreactores de tanque agitado tambi\u00e9n se utilizan principalmente en cultivos de c\u00e9lulas en suspensi\u00f3n. A continuaci\u00f3n, se indican algunos de los principales escenarios de aplicaci\u00f3n:<\/p>\n

        \n
      1. Crecimiento celular: Los biorreactores de tanque agitado se utilizan a menudo en la etapa de crecimiento celular del proceso de cultivo celular. A trav\u00e9s del sistema de agitaci\u00f3n y ventilaci\u00f3n, se pueden proporcionar suficientes nutrientes y ox\u00edgeno a las c\u00e9lulas, y las c\u00e9lulas se pueden mantener en suspensi\u00f3n para promover el crecimiento y la reproducci\u00f3n celular.<\/li>\n
      2. Expresi\u00f3n de prote\u00ednas: En la expresi\u00f3n de prote\u00ednas, los biorreactores de tanque agitado se pueden utilizar en cultivos celulares y procesos de expresi\u00f3n de prote\u00ednas. Al regular par\u00e1metros como la velocidad de agitaci\u00f3n, la temperatura y los nutrientes, se pueden optimizar el crecimiento celular y los niveles de expresi\u00f3n de prote\u00ednas.<\/li>\n
      3. Producci\u00f3n de vacunas: Los biorreactores de tanque agitado se utilizan ampliamente en el proceso de producci\u00f3n de vacunas. Al optimizar las condiciones de agitaci\u00f3n y cultivo, se puede mejorar el rendimiento y la calidad de las vacunas y se puede garantizar la estabilidad de los ingredientes activos en las vacunas.<\/li>\n
      4. Producci\u00f3n de antibi\u00f3ticos: En el proceso de producci\u00f3n de antibi\u00f3ticos, se utilizan biorreactores de tanque agitado para el cultivo y la fermentaci\u00f3n de microorganismos. Al agitar y controlar la temperatura de reacci\u00f3n, el valor de pH y otras condiciones, se puede aumentar la tasa de crecimiento de las cepas microbianas y el rendimiento de los antibi\u00f3ticos.<\/li>\n
      5. Producci\u00f3n de productos cosm\u00e9ticos: Los biorreactores de tanque agitado se utilizan para los pasos de mezcla y reacci\u00f3n en la producci\u00f3n de productos cosm\u00e9ticos. Al agitar y controlar las condiciones de reacci\u00f3n, es posible garantizar que las materias primas se mezclen por completo y se mejore la calidad del producto.<\/li>\n<\/ol>\n

        La aplicaci\u00f3n de biorreactores de tanque agitado en cultivos de c\u00e9lulas en suspensi\u00f3n puede proporcionar a las c\u00e9lulas un buen entorno de crecimiento y condiciones de reacci\u00f3n optimizadas para mejorar la eficiencia de la producci\u00f3n y la calidad del producto.<\/p>","protected":false},"excerpt":{"rendered":"

        The stirred tank bioreactor is one of the most common suspended cell culture bioreactors. It keeps the cells in the culture in a suspended state by mechanical stirring and provides sufficient oxygen and nutrients for cell growth. The structure of the stirred tank bioreactor is relatively simple and easy to operate and maintain. However, the […]<\/p>\n","protected":false},"author":1,"featured_media":3873,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[],"class_list":["post-3871","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bailun"],"_links":{"self":[{"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/posts\/3871","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/comments?post=3871"}],"version-history":[{"count":3,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/posts\/3871\/revisions"}],"predecessor-version":[{"id":5459,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/posts\/3871\/revisions\/5459"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/media\/3873"}],"wp:attachment":[{"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/media?parent=3871"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/categories?post=3871"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/tags?post=3871"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}