{"id":3894,"date":"2024-09-10T09:12:22","date_gmt":"2024-09-10T09:12:22","guid":{"rendered":"https:\/\/fermentorchina.com\/?p=3894"},"modified":"2025-04-17T09:39:07","modified_gmt":"2025-04-17T09:39:07","slug":"bioreactor-system-with-metabolic-flow-analysis","status":"publish","type":"post","link":"https:\/\/fermentorchina.com\/es\/bioreactor-system-with-metabolic-flow-analysis\/","title":{"rendered":"Sistema de biorreactor con an\u00e1lisis de flujo metab\u00f3lico como n\u00facleo"},"content":{"rendered":"

1\u3001 Principios y composici\u00f3n del dise\u00f1o del sistema<\/b><\/strong><\/h2>\n

A biological process is the process in which a biological system undergoes biochemical reactions in a bioreactor. The bioreactor system\u00a0and its surrounding environment form a relatively closed ecosystem. In this ecosystem, there are interactions between biological systems and environmental factors.<\/p>\n

\"5L
Sistema de biorreactor totalmente autom\u00e1tico de flujo metab\u00f3lico cu\u00e1druple de 5 l<\/a><\/figcaption><\/figure>\n

The phenotype of biological systems, especially those dominated by cells, is closely related not only to genotype, but also to the micro or macro environmental conditions in which the cells are located (such as nutrient types, pH, temperature, dissolved oxygen, mixing and transfer characteristics of bioreactors, etc.). In other words, genotype and environment jointly determine the phenotypic characteristics of biological systems. On the other hand, biological processes have highly nonlinear and time-varying characteristics, making it difficult to characterize their complex ontological features using macroscopic dynamics aimed at detecting and controlling environmental operating parameters.<\/p>\n

Therefore, it is necessary to systematically analyze the metabolic changes of cells based on the metabolic characteristics related to parameters during the fermentation process, emphasizing that the physiological state of cells is related to parameters and is the result of the transfer, conversion, and balance of materials, energy, or information in bioreactors. Although its micro influencing factors may only occur at a certain scale at the level of genes, enzymes, cells, or reactors, they will ultimately be reflected in macro processes, providing clues for studying data correlation analysis methods at different scales in bioreactors.<\/p>\n

\"10L
Sistema de biorreactor totalmente automatizado de triple metabolismo de 10 l<\/a><\/figcaption><\/figure>\n

An ideal bioreactor system\u00a0should detect and analyze operating parameters and state parameters at different scales as much as possible, so as to potentially construct an optimized external environment that maximizes the gene expression and metabolic regulation of microorganisms for the biosynthesis of a certain target product and maximizes the accumulation of the target product.<\/p>\n

The above indicates the engineering science issues of biological processes, which have evolved from macroscopic dynamics research to multi-scale theoretical methods based on biological process information processing, in order to guide the development of engineering technologies related to biological processes with bioreactor system\u00a0as the core.<\/p>\n

Bailun adopts a metabolic flow multi-scale biological reaction system, which includes various advanced sensors for the detection of cell physiological metabolic characteristics, sensor reactors and control systems for micro metabolic flow analysis of biological processes, computer software packages for the correlation analysis of cell physiological metabolic parameters, and computer Internet systems for data processing and remote analysis of fermentation processes.<\/p>\n

This system is consistent with the highest configuration in Europe, reducing the interference of human factors on scientific research, improving data repeatability and accuracy, reducing labor and material costs, without the need for dedicated personnel on duty, and can achieve computer remote control and wireless monitoring.<\/p>\n

\"10L-10L-10L-50L
10L-10L-10L-50L metabolic flow fully automatic bioreactor system<\/figcaption><\/figure>\n

The can lid automatic opening system, and all parameters can be automatically controlled. Being able to obtain as much biological information as possible at various scales of the biological processing process, and then based on the principle of multi-scale parameter correlation, through real-time data processing of computer software, identifying key parameters for process optimization based on parameter correlation characteristics in massive data, and using them to guide process operations, equipment design, or strain screening and transformation, ultimately achieving process optimization and scaling up.<\/p>\n

The system has been successfully applied to optimize the production process of various products, greatly improving the capacity of fermentation units. Its optimized process can generally be directly scaled up from tens of liters of fermentation tanks to industrial production fermentation tanks of hundreds of cubic meters.<\/p>\n

<\/b>2. Desarrollo y aplicaci\u00f3n de instrumentos avanzados en l\u00ednea<\/b><\/strong><\/h2>\n

(1)Sensores en l\u00ednea<\/b><\/strong>\u00a0<\/b><\/strong>del sistema de biorreactor<\/b><\/strong><\/h3>\n

The bioreactor system\u00a0with metabolic flow analysis as its core is equipped with advanced sensing systems. In addition to conventional detection and control parameters such as pH, temperature, stirring speed, dissolved oxygen, and rotor flow meter, a thermal mass flow meter (for precise measurement and control of intake flow rate to ensure it is not affected by intake pressure), a fermentation broth weighing system, exhaust gas oxygen and CO2 measuring instruments (exhaust gas composition analyzer or process exhaust gas mass spectrometer), and a top mounted silicone oil pressure sensor are also configured to accurately measure the oxygen consumption rate (OUR) and CO2 release rate (CER) of biological processes.<\/p>\n

Micro metabolic flow analysis sensor reactors and control systems, in situ live cell concentration online measuring instruments, cell morphology online microscopic observation instruments, and other online sensors can also be configured as needed. Based on the direct parameters obtained from the measurement, important physiological and metabolic state parameters such as CER, OUR, and RQ can be calculated using computer software packages for cell physiological and metabolic parameter analysis.<\/p>\n

\"Magnetic
Magnetic driving glass fermenter 5 liter BLBIO-5GC<\/a><\/figcaption><\/figure>\n

\"Microbial\"Microbial<\/p>\n

(2)Espectr\u00f3metro de masas de gases de escape de <\/b><\/strong>sistema de biorreactor<\/b><\/strong><\/h3>\n

The process exhaust gas mass spectrometer is mainly used for measuring the concentrations of O2, CO2, and N2 in exhaust gas. This mass spectrometer uses an electron bombardment ion source. After online pretreatment, the exhaust gases from different fermentation tanks are continuously input into the ionization chamber through a multi-channel rotary valve to form ions.<\/p>\n

Using the motion law of charged particles in the electric field, the quadrupole mass analyzer separates the ions generated by the ion source according to their mass to charge ratio (mass to charge ratio, m\/z), measures the distribution of ion mass intensity, and obtains information on compound types and concentrations, accurately reflecting changes in the composition of fermentation exhaust gases.<\/p>\n

The measurement results can be input into a specialized software package designed for the fermentation process, enabling correlation analysis with other fermentation parameters. The exhaust gas mass spectrometer can measure volatile gas components with a relative molecular weight of up to 300, so it can also detect small molecule substances such as ethanol and methanol according to fermentation needs.<\/p>\n

(3)Analizador de medidas <\/b><\/strong>de <\/b><\/strong>sistema de biorreactor<\/b><\/strong><\/h3>\n

Biomass is an important parameter in the fermentation process. At present, it is usually obtained through offline measurements using classic methods such as dry mass and turbidity. The in-situ live cell concentration online analyzer can not only perform real-time online measurement, but also obtain more biologically meaningful live cell concentrations.<\/p>\n

This is particularly suitable for fermentation processes that contain insoluble solid substances in the culture medium. The principle of the live cell analyzer is based on 0 In an alternating electric field with a frequency range of 1-10MHz, non-conductive polarization occurs on the cell surface such as the cell membrane in the fermentation broth, making living cells with intact cytoplasmic membranes essentially like capacitors (the non-conductive nature of lipid cytoplasmic membranes generally leads to charge growth), while dead cells, lysed cells, cell fragments, bubbles, and other matrix components are essentially non polarizable.<\/p>\n

By applying an alternating electric field within the frequency range using dual electrodes, the measured capacitance between the electrodes depends on the cell type and size, and is proportional to the concentration of viable bacteria within a certain range. The in situ live cell concentration online analyzer is applicable to various animal and plant cells, yeast, bacteria, algae, etc. But it is not suitable for fermentation processes that require acid-base neutralizing agents as the target product, as excessive ion strength in the fermentation broth can interfere with the accurate measurement of capacitance.<\/p>\n

Microbiorreactor<\/b><\/strong><\/h2>\n

The micro bioreactor system\u00a0is a novel biochemical experimental platform that enables the study and control of biological reactions in small-scale reactors. This system combines the advantages of microfluidic technology, microelectronic devices, and biotechnology to perform the functions of traditional bioreactors at the microscale. Micro bioreactor systems\u00a0typically consist of microfluidic chips, micro pumps, temperature control, and control systems. Microfluidic chips are core components composed of microchannels and microvalves, used to regulate the flow and mixing of microorganisms and substrates in reactors.<\/p>\n

\"Mini
Mini glass bioreactor 1GJ-4 waterless temperature-controlled fermenter<\/a><\/figcaption><\/figure>\n

The micro pump is responsible for providing fluid pumping and controlling flow rate, the temperature control system is used to maintain a constant temperature inside the reactor, and the control system is used to monitor and regulate the progress of the reaction in real time. The micro bioreactor system\u00a0has many advantages. Firstly, due to the small size of the reactor, it can reduce reaction time and substrate consumption, thereby improving reaction efficiency. Secondly, microfluidic chips can achieve precise control of reaction parameters such as temperature, pH value, and substrate concentration, making experimental results more accurate and reliable.<\/p>\n

In addition, due to the small size of the reactor, multiple reactors can be run in parallel on the same experimental platform for high-throughput experiments, accelerating the progress of scientific research. Micro bioreactor systems\u00a0have broad application prospects in fields such as biomedical, biopharmaceutical, environmental protection, and food industry. Through the application of micro bioreactor systems, researchers can delve deeper into the physiological characteristics of microorganisms, accelerate the development of new drugs, improve the efficiency of biological production processes, and develop new treatment methods and environmental monitoring methods.<\/p>\n

Micro <\/b><\/strong>sistemas de biorreactores<\/b><\/strong>\u00a0Por lo general, constan de los siguientes componentes principales:<\/b><\/strong><\/h2>\n

(1) Chip microflu\u00eddico de <\/b><\/strong>sistema de biorreactor<\/b><\/strong>: <\/b><\/strong>El chip microflu\u00eddico es el componente principal del sistema de microbiorreactor. Consta de una red de canales a nivel microm\u00e9trico y microv\u00e1lvulas. Al ajustar la apertura y el cierre de las microv\u00e1lvulas, se puede controlar el flujo y la mezcla de fluidos en los microcanales, logrando un control preciso de las condiciones de reacci\u00f3n. Los chips microflu\u00eddicos suelen estar hechos de vidrio, pol\u00edmero o materiales a base de silicio.<\/p>\n

(2) Microbomba <\/b><\/strong>de <\/b><\/strong>sistema de biorreactor<\/b><\/strong>: <\/b><\/strong>Una microbomba es un dispositivo que se utiliza para bombear fluidos. Las microbombas suelen accionarse por presi\u00f3n o por medios el\u00e9ctricos, lo que permite controlar con precisi\u00f3n los fluidos. Puede introducir sustratos, medios de cultivo, reactivos, etc. en chips microflu\u00eddicos para controlar el caudal y el grado de mezcla de fluidos dentro del reactor.<\/p>\n

(3) Sistema de control de temperatura <\/b><\/strong>de <\/b><\/strong>sistema de biorreactor<\/b><\/strong>:<\/b><\/strong>\u00a0El sistema de control de temperatura se utiliza para mantener una temperatura constante dentro del reactor. El control preciso de la temperatura del reactor se puede lograr mediante elementos de calentamiento y sensores. La temperatura es crucial para el crecimiento y el metabolismo de los microorganismos, por lo tanto, la estabilidad de los sistemas de control de temperatura es esencial para la precisi\u00f3n de los resultados experimentales.<\/p>\n

(4) Sistema de control <\/b><\/strong>de <\/b><\/strong>sistema de biorreactor<\/b><\/strong>: <\/b><\/strong>Un sistema de control generalmente consta de hardware y software, que se utilizan para el monitoreo y la regulaci\u00f3n en tiempo real del funcionamiento de un sistema de microbiorreactor. Puede monitorear par\u00e1metros como la temperatura, el caudal, la presi\u00f3n, etc., y ajustarlos autom\u00e1ticamente seg\u00fan las condiciones de reacci\u00f3n preestablecidas. El sistema de control tambi\u00e9n puede registrar datos durante el proceso experimental y realizar an\u00e1lisis y procesamiento de datos.<\/p>\n

In addition to the main components mentioned above, the micro bioreactor system\u00a0may also include additional components such as sensors, detection instruments, and data storage devices. Sensors can monitor indicators such as biological activity, substrate concentration, and product generation in the reactor. Detection instruments are used to analyze and measure the quality and quality of the reaction process, while data storage devices are used to store experimental data and results.<\/p>\n

The micro bioreactor system\u00a0achieves precise control and efficient experimentation of microbial reaction processes through the combination of microfluidic chips and related equipment. This system has broad application potential in fields such as life sciences, drug development, and industrial production.<\/p>\n

El micro <\/b><\/strong>sistema de biorreactor<\/b><\/strong>\u00a0Tiene las siguientes ventajas:<\/b><\/strong><\/h2>\n

(1) Tama\u00f1o peque\u00f1o: <\/b><\/strong>El tama\u00f1o de los sistemas de microbiorreactores es peque\u00f1o, y suele oscilar entre micr\u00f3metros y mil\u00edmetros. En comparaci\u00f3n con los sistemas de biorreactores tradicionales, los microrreactores tienen un volumen menor, lo que permite ahorrar espacio en el laboratorio y el funcionamiento simult\u00e1neo de varios reactores en dispositivos relativamente peque\u00f1os. Este dise\u00f1o de tama\u00f1o reducido tambi\u00e9n puede reducir la cantidad de muestras y reactivos utilizados, lo que reduce los costos experimentales.<\/p>\n

(2) Velocidad de reacci\u00f3n r\u00e1pida: <\/b><\/strong>Debido a su peque\u00f1o volumen, los microrreactores tienen un volumen de reacci\u00f3n menor, lo que permite que los reactivos se mezclen y difundan m\u00e1s r\u00e1pidamente en el canal, lo que aumenta la eficiencia de la reacci\u00f3n para acelerar la velocidad de reacci\u00f3n. Por lo tanto, los sistemas de microbiorreactores pueden acortar el tiempo de reacci\u00f3n, acelerar los procesos experimentales y mejorar la eficiencia experimental.<\/p>\n

(3) Bajo consumo de muestra y reactivo:<\/b><\/strong>\u00a0El volumen del reactor en el sistema de microbiorreactor es relativamente peque\u00f1o, lo que da como resultado una reducci\u00f3n correspondiente en la cantidad requerida de muestras y reactivos. Esto no solo ahorra costos de muestras y reactivos costosos, sino que tambi\u00e9n ayuda a conservar muestras biol\u00f3gicas escasas y reactivos valiosos en la investigaci\u00f3n.<\/p>\n

(4) Control preciso de par\u00e1metros: <\/b><\/strong>Los chips microflu\u00eddicos desempe\u00f1an un papel crucial en los sistemas de microbiorreactores. Mediante el control de microcanales y microv\u00e1lvulas, se puede lograr un control preciso de par\u00e1metros como el flujo de reactivos, la temperatura, la presi\u00f3n y la mezcla. Este control preciso puede hacer que los resultados experimentales sean m\u00e1s precisos y confiables, y brindar m\u00e1s posibilidades para optimizar las condiciones de reacci\u00f3n biol\u00f3gica.<\/p>\n

(5) Experimentos de alto rendimiento: <\/b><\/strong>Los sistemas de microbiorreactores pueden lograr el funcionamiento en paralelo de m\u00faltiples reactores, conocidos como experimentos de alto rendimiento. En la misma plataforma experimental, se pueden probar m\u00faltiples condiciones experimentales simult\u00e1neamente, lo que mejora enormemente la eficiencia del trabajo y el rendimiento experimental de los investigadores. Los experimentos de alto rendimiento tienen un valor de aplicaci\u00f3n importante en la detecci\u00f3n de f\u00e1rmacos, el an\u00e1lisis de la expresi\u00f3n gen\u00e9tica y otras investigaciones.<\/p>\n

(6) Integraci\u00f3n y automatizaci\u00f3n:<\/b><\/strong>\u00a0Los sistemas de microbiorreactores se pueden integrar con otros dispositivos e instrumentos para lograr operaciones automatizadas. Al conectarlos y coordinarlos con sistemas de control de temperatura, sistemas de procesamiento de l\u00edquidos, instrumentos de detecci\u00f3n, etc., se puede lograr un control del proceso de reacci\u00f3n y un procesamiento de datos totalmente automatizados, mejorando la repetibilidad y la estabilidad de los experimentos.<\/p>\n

(7) El micro <\/b><\/strong>sistema de biorreactor<\/b><\/strong>\u00a0tiene las ventajas de <\/b><\/strong>Su peque\u00f1o tama\u00f1o, su r\u00e1pida velocidad de reacci\u00f3n, su bajo consumo de muestras y reactivos, su control preciso de par\u00e1metros, sus experimentos de alto rendimiento y su automatizaci\u00f3n lo convierten en una herramienta importante y ampliamente utilizada en campos como la biotecnolog\u00eda, las ciencias biol\u00f3gicas y la investigaci\u00f3n y producci\u00f3n de f\u00e1rmacos.<\/p>\n

Micro<\/b><\/strong>\u00a0sistemas de biorreactores<\/b><\/strong>\u00a0Tienen una amplia gama de aplicaciones en muchos campos. A continuaci\u00f3n se indican algunas \u00e1reas de aplicaci\u00f3n importantes:<\/b><\/strong><\/h2>\n

(1) Detecci\u00f3n y desarrollo de f\u00e1rmacos: <\/b><\/strong>Los sistemas de microbiorreactores se utilizan ampliamente en los procesos de detecci\u00f3n y desarrollo de f\u00e1rmacos. Al cultivar c\u00e9lulas en chips microflu\u00eddicos, se puede evaluar la toxicidad y la eficacia de los f\u00e1rmacos. El peque\u00f1o tama\u00f1o y las caracter\u00edsticas de alto rendimiento de los microrreactores permiten analizar varias muestras de f\u00e1rmacos simult\u00e1neamente, lo que acelera enormemente la velocidad de detecci\u00f3n de f\u00e1rmacos. Adem\u00e1s, el sistema de microbiorreactores puede simular el metabolismo y la acci\u00f3n de los f\u00e1rmacos in vivo, lo que proporciona una evaluaci\u00f3n m\u00e1s precisa de la eficacia de los f\u00e1rmacos y una optimizaci\u00f3n de la dosis.<\/p>\n

(2) Biofarmac\u00e9uticos: <\/b><\/strong>Los sistemas de microbiorreactores pueden desempe\u00f1ar un papel importante en los procesos biofarmac\u00e9uticos. Al cultivar f\u00e1bricas de c\u00e9lulas en chips microflu\u00eddicos, se puede lograr una producci\u00f3n y purificaci\u00f3n eficientes de medicamentos biol\u00f3gicos. Las capacidades precisas de control de temperatura y fluidos de los microrreactores pueden optimizar las condiciones de reacci\u00f3n biol\u00f3gica, mejorar la eficiencia de la producci\u00f3n y la calidad de los resultados, y reducir los costos de producci\u00f3n.<\/p>\n

(3) Investigaci\u00f3n microbiana:<\/b><\/strong>\u00a0Los sistemas de microbiorreactores se utilizan ampliamente en la investigaci\u00f3n microbiana. Las caracter\u00edsticas fisiol\u00f3gicas y las v\u00edas metab\u00f3licas de los microorganismos se pueden simular y analizar en chips microflu\u00eddicos, revelando los procesos biol\u00f3gicos b\u00e1sicos de los microorganismos. Los investigadores pueden estudiar los mecanismos de respuesta y adaptaci\u00f3n de los microorganismos a diferentes condiciones ambientales controlando con precisi\u00f3n los efectos de diferentes factores como la temperatura, el valor del pH, la concentraci\u00f3n de ox\u00edgeno, etc. Estos estudios pueden ayudar a profundizar nuestra comprensi\u00f3n de la ecolog\u00eda, las v\u00edas metab\u00f3licas y los mecanismos evolutivos de los microorganismos.<\/p>\n

(4) Monitoreo ambiental:<\/b><\/strong>\u00a0Los sistemas de microbiorreactores se pueden utilizar para la monitorizaci\u00f3n ambiental y la detecci\u00f3n de contaminantes. A trav\u00e9s de chips microflu\u00eddicos, se pueden cultivar cepas microbianas espec\u00edficas para detectar sustancias t\u00f3xicas, metales pesados y contaminantes en el medio ambiente. La sensibilidad y la r\u00e1pida velocidad de reacci\u00f3n de los microrreactores a la actividad microbiana los convierten en una herramienta de monitorizaci\u00f3n ambiental eficiente, precisa y en tiempo real.<\/p>\n

(5) Investigaci\u00f3n en ciencias de la vida:<\/b><\/strong>\u00a0Los sistemas de microbiorreactores tambi\u00e9n se han utilizado ampliamente en la investigaci\u00f3n de las ciencias biol\u00f3gicas. Los investigadores pueden utilizar sistemas de microbiorreactores para estudiar procesos biol\u00f3gicos como la se\u00f1alizaci\u00f3n celular, la expresi\u00f3n gen\u00e9tica, la s\u00edntesis de prote\u00ednas y las v\u00edas metab\u00f3licas. Al controlar con precisi\u00f3n las condiciones de reacci\u00f3n, podemos comprender mejor las interacciones entre biomol\u00e9culas, funciones celulares y mecanismos de desarrollo de enfermedades.<\/p>\n

La aplicaci\u00f3n de los sistemas de microbiorreactores abarca una amplia gama de campos, como la detecci\u00f3n de f\u00e1rmacos, los productos biofarmac\u00e9uticos, la investigaci\u00f3n microbiol\u00f3gica, el control medioambiental y la investigaci\u00f3n en ciencias de la vida. Las caracter\u00edsticas de alto rendimiento, control preciso y respuesta r\u00e1pida de los sistemas de biorreactores proporcionan a los investigadores una plataforma experimental eficiente, fiable e innovadora.<\/p>","protected":false},"excerpt":{"rendered":"

1\u3001 System design principles and composition A biological process is the process in which a biological system undergoes biochemical reactions in a bioreactor. The bioreactor system\u00a0and its surrounding environment form a relatively closed ecosystem. In this ecosystem, there are interactions between biological systems and environmental factors. The phenotype of biological systems, especially those dominated by […]<\/p>\n","protected":false},"author":1,"featured_media":3896,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[],"class_list":["post-3894","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bailun"],"_links":{"self":[{"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/posts\/3894","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/comments?post=3894"}],"version-history":[{"count":6,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/posts\/3894\/revisions"}],"predecessor-version":[{"id":5462,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/posts\/3894\/revisions\/5462"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/media\/3896"}],"wp:attachment":[{"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/media?parent=3894"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/categories?post=3894"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fermentorchina.com\/es\/wp-json\/wp\/v2\/tags?post=3894"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}