{"id":3871,"date":"2024-08-07T08:56:47","date_gmt":"2024-08-07T08:56:47","guid":{"rendered":"https:\/\/fermentorchina.com\/?p=3871"},"modified":"2025-04-17T09:36:22","modified_gmt":"2025-04-17T09:36:22","slug":"suspended-cell-culture-bioreactor-stirred-tank-bioreactor","status":"publish","type":"post","link":"https:\/\/fermentorchina.com\/ru\/suspended-cell-culture-bioreactor-stirred-tank-bioreactor\/","title":{"rendered":"\u0411\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440 \u0441 \u0441\u0443\u0441\u043f\u0435\u043d\u0437\u0438\u043e\u043d\u043d\u044b\u043c\u0438 \u043a\u043b\u0435\u0442\u043e\u0447\u043d\u044b\u043c\u0438 \u043a\u0443\u043b\u044c\u0442\u0443\u0440\u0430\u043c\u0438: \u0411\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440 \u0441 \u043c\u0435\u0448\u0430\u043b\u043a\u043e\u0439"},"content":{"rendered":"

The stirred tank bioreactor is one of the most common suspended cell culture bioreactors. It keeps the cells in the culture in a suspended state by mechanical stirring and provides sufficient oxygen and nutrients for cell growth.<\/p>\n

\"300L-animal-cell-bioreactor-BLBIO-300SCUC
300L-animal-cell-bioreactor-BLBIO-300SCUC<\/a><\/figcaption><\/figure>\n

The structure of the stirred tank bioreactor is relatively simple and easy to operate and maintain. However, the shear force and bubble impact caused by stirring on the cells may have an adverse effect on cell growth and product yield. Therefore, the stirring speed and gas intake need to be controlled in the stirred tank bioreactor to balance the mixing of the culture and the gas-liquid mass transfer process.<\/p>\n

\"\"\u041f\u043e \u0441\u0440\u0430\u0432\u043d\u0435\u043d\u0438\u044e \u0441 \u0431\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u0430\u043c\u0438 \u0441 \u043c\u0435\u0448\u0430\u043b\u043a\u043e\u0439, <\/span>\u0431\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u044b \u0441 \u0432\u043e\u0437\u0434\u0443\u0448\u043d\u044b\u043c \u043f\u043e\u0434\u044a\u0435\u043c\u043e\u043c have better performance in the gas-liquid mass transfer process. Airlift bioreactors achieve gas-liquid mass transfer through the rising movement of bubbles, and the contact area between bubbles and liquid phase is greatly increased. <\/span><\/p>\n

In addition, the liquid flow generated by the rising movement of gas in the airlift bioreactor also helps to mix the suspended cells and reduce the differences in the local liquid environment. Therefore, the airlift bioreactor can provide better oxygen and nutrient supply while reducing the shear force on the suspended cells. However, the structure of the airlift bioreactor is relatively complex, and the operation and maintenance are difficult, requiring special gas devices and control systems.<\/span><\/p>\n

In recent years, the application of single use bioreactors has gradually increased, becoming an emerging suspended cell culture bioreactor. Due to its relatively simple structure and operation, single use bioreactors reduce the workload of reactor cleaning and disinfection, can reduce the risk of cross infection, and save production costs.<\/p>\n

\"Bailun
Bailun 300L mammalian cell culture bioreactors BLBIO-300SCUC<\/figcaption><\/figure>\n

Single use bioreactors also have good scalability and are convenient for mass production. However, single use bioreactors may have some problems in gas-liquid mass transfer and control, such as uneven supply of oxygen and nutrients, and difficulty in adjusting pH and temperature. Therefore, when using single use bioreactors, improvements and optimizations need to be made according to specific circumstances.\"\"\"\"<\/p>\n

In order to better meet the needs of suspended cell culture bioreactors, researchers have continuously explored and developed various new reactor designs in practice. For example, flexible wall suspended cell culture bioreactors can provide better isolation of liquid environment and shear force on cells by changing the wall material and structure of the culture chamber.<\/p>\n

The membrane suspended cell culture bioreactor separates the liquid phase and gas phase through the support of the membrane, reducing the impact and shear force of bubbles on cells. These new reactor designs provide new ideas and directions for the development of suspended cell culture bioreactors.\"\"<\/p>\n

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\"glass
Bailun mammalian cell culture reactors single wall glass bioreactor<\/figcaption><\/figure>\n

The stirred tank bioreactor is a common suspended cell culture bioreactor, which is widely used in biopharmaceuticals, biofuels and other bioprocesses. It keeps the cells in the culture in a suspended state by mechanical stirring and provides sufficient oxygen and nutrients for cell growth. The basic structure of the stirred tank bioreactor includes a tank body, a stirring device, an air inlet device, a liquid level control device, a temperature control device, etc.<\/p>\n

The tank body of the stirred tank bioreactor is usually made of stainless steel, which has high corrosion resistance and chemical stability. The stirring device of the stirred tank bioreactor is generally composed of a motor and a stirring paddle, and the intensity and speed of stirring can be controlled by adjusting the speed.<\/p>\n

The air inlet device of the stirred tank bioreactor is used to inject gas, usually air or pure oxygen, into the culture medium to provide oxygen for cell growth. The liquid level control device of the stirred tank bioreactor is used to monitor and control the liquid level of the culture medium to maintain the appropriate volume and mixing degree of the culture medium. The temperature control device of the stirred tank bioreactor is used to maintain the temperature of the culture medium within an appropriate range.<\/p>\n

In a stirred tank bioreactor, the suspension state of cells is usually achieved by the turbulent flow generated by the stirring device. The shape and stirring speed of the stirring device have an important influence on the suspension of cells and the mixing effect of the liquid. Generally speaking, in the process of suspended cell culture, a moderate stirring intensity is required to maintain the suspension state of cells, but too high a stirring speed may cause excessive shear force on the cells, which will have an adverse effect on the integrity and biological activity of the cells.<\/p>\n

Therefore, when using a stirred tank bioreactor, it is necessary to determine the appropriate stirring speed according to the specific cell type and culture conditions.<\/p>\n

\"20L-cell-culture
20L-cell-culture bioreactor BLBIO-20GJC mammalian cell culture bioreactor<\/figcaption><\/figure>\n

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\u0412 \u0441\u0443\u0441\u043f\u0435\u043d\u0437\u0438\u043e\u043d\u043d\u043e\u0439 \u043a\u0443\u043b\u044c\u0442\u0443\u0440\u0435 \u043a\u043b\u0435\u0442\u043e\u043a \u0431\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u044b \u0441 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u0435\u043c\u044b\u043c \u0440\u0435\u0437\u0435\u0440\u0432\u0443\u0430\u0440\u043e\u043c \u0438\u043c\u0435\u044e\u0442 \u0441\u043b\u0435\u0434\u0443\u044e\u0449\u0438\u0435 \u043f\u0440\u0435\u0438\u043c\u0443\u0449\u0435\u0441\u0442\u0432\u0430 \u0438 \u043d\u0435\u0434\u043e\u0441\u0442\u0430\u0442\u043a\u0438:<\/p>\n

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    \n
  1. \u0420\u0430\u0432\u043d\u043e\u043c\u0435\u0440\u043d\u043e\u0435 \u0441\u043c\u0435\u0448\u0438\u0432\u0430\u043d\u0438\u0435: \u0411\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440 \u0441 \u043c\u0435\u0448\u0430\u043b\u043a\u043e\u0439 \u0440\u0430\u0432\u043d\u043e\u043c\u0435\u0440\u043d\u043e \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u0435\u0442 \u043a\u043b\u0435\u0442\u043a\u0438, \u043a\u0443\u043b\u044c\u0442\u0443\u0440\u0430\u043b\u044c\u043d\u0443\u044e \u0441\u0440\u0435\u0434\u0443 \u0438 \u0433\u0430\u0437 \u0432 \u043a\u0443\u043b\u044c\u0442\u0443\u0440\u0430\u043b\u044c\u043d\u043e\u0439 \u0441\u0440\u0435\u0434\u0435 \u0441 \u043f\u043e\u043c\u043e\u0449\u044c\u044e \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u044e\u0449\u0435\u0433\u043e \u0443\u0441\u0442\u0440\u043e\u0439\u0441\u0442\u0432\u0430, \u0431\u043b\u0430\u0433\u043e\u0434\u0430\u0440\u044f \u0447\u0435\u043c\u0443 \u043a\u043b\u0435\u0442\u043a\u0438 \u043c\u043e\u0433\u0443\u0442 \u043f\u043e\u043b\u043d\u043e\u0441\u0442\u044c\u044e \u043a\u043e\u043d\u0442\u0430\u043a\u0442\u0438\u0440\u043e\u0432\u0430\u0442\u044c \u0441 \u043f\u0438\u0442\u0430\u0442\u0435\u043b\u044c\u043d\u044b\u043c\u0438 \u0432\u0435\u0449\u0435\u0441\u0442\u0432\u0430\u043c\u0438 \u0438 \u043a\u0438\u0441\u043b\u043e\u0440\u043e\u0434\u043e\u043c \u0432 \u043a\u0443\u043b\u044c\u0442\u0443\u0440\u0430\u043b\u044c\u043d\u043e\u0439 \u0441\u0440\u0435\u0434\u0435, \u0447\u0442\u043e \u0441\u043f\u043e\u0441\u043e\u0431\u0441\u0442\u0432\u0443\u0435\u0442 \u0440\u043e\u0441\u0442\u0443 \u043a\u043b\u0435\u0442\u043e\u043a \u0438 \u0432\u044b\u0440\u0430\u0431\u043e\u0442\u043a\u0435 \u043c\u0435\u0442\u0430\u0431\u043e\u043b\u0438\u0442\u043e\u0432.<\/li>\n
  2. \u0420\u0435\u0433\u0443\u043b\u0438\u0440\u0443\u0435\u043c\u044b\u0435 \u0443\u0441\u043b\u043e\u0432\u0438\u044f \u0440\u0435\u0430\u043a\u0446\u0438\u0438: \u0440\u0435\u0433\u0443\u043b\u0438\u0440\u0443\u044f \u0441\u043a\u043e\u0440\u043e\u0441\u0442\u044c \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u043d\u0438\u044f, \u0442\u0435\u043c\u043f\u0435\u0440\u0430\u0442\u0443\u0440\u0443, \u0437\u043d\u0430\u0447\u0435\u043d\u0438\u0435 pH \u0438 \u0434\u0440\u0443\u0433\u0438\u0435 \u043f\u0430\u0440\u0430\u043c\u0435\u0442\u0440\u044b, \u043c\u043e\u0436\u043d\u043e \u043a\u043e\u043d\u0442\u0440\u043e\u043b\u0438\u0440\u043e\u0432\u0430\u0442\u044c \u0443\u0441\u043b\u043e\u0432\u0438\u044f \u0440\u0435\u0430\u043a\u0446\u0438\u0438 \u0432 \u043f\u0440\u043e\u0446\u0435\u0441\u0441\u0435 \u043a\u0443\u043b\u044c\u0442\u0438\u0432\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f \u043a\u043b\u0435\u0442\u043e\u043a \u0434\u043b\u044f \u0443\u0434\u043e\u0432\u043b\u0435\u0442\u0432\u043e\u0440\u0435\u043d\u0438\u044f \u043f\u043e\u0442\u0440\u0435\u0431\u043d\u043e\u0441\u0442\u0435\u0439 \u0440\u043e\u0441\u0442\u0430 \u0438 \u044d\u043a\u0441\u043f\u0440\u0435\u0441\u0441\u0438\u0438 \u0440\u0430\u0437\u043b\u0438\u0447\u043d\u044b\u0445 \u043a\u043b\u0435\u0442\u043e\u043a.<\/li>\n
  3. \u041f\u0440\u043e\u0441\u0442\u043e\u0442\u0430 \u044d\u043a\u0441\u043f\u043b\u0443\u0430\u0442\u0430\u0446\u0438\u0438 \u0438 \u043a\u043e\u043d\u0442\u0440\u043e\u043b\u044f: \u0411\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440 \u0441 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u044e\u0449\u0438\u043c \u0440\u0435\u0437\u0435\u0440\u0432\u0443\u0430\u0440\u043e\u043c \u043f\u0440\u043e\u0441\u0442 \u043f\u043e \u043a\u043e\u043d\u0441\u0442\u0440\u0443\u043a\u0446\u0438\u0438 \u0438 \u043f\u0440\u043e\u0441\u0442 \u0432 \u044d\u043a\u0441\u043f\u043b\u0443\u0430\u0442\u0430\u0446\u0438\u0438. \u041e\u043d \u043c\u043e\u0436\u0435\u0442 \u043a\u043e\u043d\u0442\u0440\u043e\u043b\u0438\u0440\u043e\u0432\u0430\u0442\u044c \u0438 \u0440\u0435\u0433\u0443\u043b\u0438\u0440\u043e\u0432\u0430\u0442\u044c \u0440\u0430\u0437\u043b\u0438\u0447\u043d\u044b\u0435 \u043f\u0430\u0440\u0430\u043c\u0435\u0442\u0440\u044b \u043f\u0438\u0442\u0430\u0442\u0435\u043b\u044c\u043d\u043e\u0439 \u0441\u0440\u0435\u0434\u044b, \u0442\u0430\u043a\u0438\u0435 \u043a\u0430\u043a \u0442\u0435\u043c\u043f\u0435\u0440\u0430\u0442\u0443\u0440\u0430, \u0437\u043d\u0430\u0447\u0435\u043d\u0438\u0435 pH \u0438 \u0440\u0430\u0441\u0442\u0432\u043e\u0440\u0435\u043d\u043d\u044b\u0439 \u043a\u0438\u0441\u043b\u043e\u0440\u043e\u0434, \u0432 \u0440\u0435\u0436\u0438\u043c\u0435 \u0440\u0435\u0430\u043b\u044c\u043d\u043e\u0433\u043e \u0432\u0440\u0435\u043c\u0435\u043d\u0438, \u0447\u0442\u043e\u0431\u044b \u043e\u0431\u0435\u0441\u043f\u0435\u0447\u0438\u0442\u044c \u0441\u0442\u0430\u0431\u0438\u043b\u044c\u043d\u043e\u0441\u0442\u044c \u0438 \u0443\u043f\u0440\u0430\u0432\u043b\u044f\u0435\u043c\u043e\u0441\u0442\u044c \u043f\u0440\u043e\u0446\u0435\u0441\u0441\u0430 \u043a\u0443\u043b\u044c\u0442\u0438\u0432\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f.<\/li>\n
  4. \u041f\u043e\u0434\u0445\u043e\u0434\u0438\u0442 \u0434\u043b\u044f \u043a\u0440\u0443\u043f\u043d\u043e\u043c\u0430\u0441\u0448\u0442\u0430\u0431\u043d\u043e\u0433\u043e \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0430: \u0411\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440 \u0441 \u043c\u0435\u0448\u0430\u043b\u043a\u043e\u0439 \u043f\u043e\u0437\u0432\u043e\u043b\u044f\u0435\u0442 \u043e\u0441\u0443\u0449\u0435\u0441\u0442\u0432\u043b\u044f\u0442\u044c \u043a\u0440\u0443\u043f\u043d\u043e\u043c\u0430\u0441\u0448\u0442\u0430\u0431\u043d\u043e\u0435 \u0441\u0443\u0441\u043f\u0435\u043d\u0437\u0438\u043e\u043d\u043d\u043e\u0435 \u043a\u0443\u043b\u044c\u0442\u0438\u0432\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u0435 \u043a\u043b\u0435\u0442\u043e\u043a, \u0447\u0442\u043e \u043f\u043e\u0434\u0445\u043e\u0434\u0438\u0442 \u0434\u043b\u044f \u043f\u0440\u043e\u043c\u044b\u0448\u043b\u0435\u043d\u043d\u043e\u0433\u043e \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0430 \u0438 \u043c\u043e\u0436\u0435\u0442 \u043f\u043e\u0432\u044b\u0441\u0438\u0442\u044c \u044d\u0444\u0444\u0435\u043a\u0442\u0438\u0432\u043d\u043e\u0441\u0442\u044c \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0430 \u0438 \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0438\u0442\u0435\u043b\u044c\u043d\u043e\u0441\u0442\u044c.<\/li>\n<\/ol>\n

    \u041d\u0435\u0434\u043e\u0441\u0442\u0430\u0442\u043a\u0438<\/b><\/strong>\u00a0<\/b><\/strong>\u0411\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440 \u0441 \u043c\u0435\u0448\u0430\u043b\u043a\u043e\u0439<\/b><\/strong>:<\/b><\/strong><\/h2>\n
      \n
    1. \u041d\u0435\u0440\u0430\u0432\u043d\u043e\u043c\u0435\u0440\u043d\u044b\u0439 \u043c\u0430\u0441\u0441\u043e\u043f\u0435\u0440\u0435\u043d\u043e\u0441 \u0433\u0430\u0437-\u0436\u0438\u0434\u043a\u043e\u0441\u0442\u044c: \u041f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u043d\u0438\u0435 \u0432 \u0431\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u0435 \u0441 \u043c\u0435\u0448\u0430\u043b\u043a\u043e\u0439 \u043f\u0440\u0438\u0432\u0435\u0434\u0435\u0442 \u043a \u0432\u043e\u0437\u043d\u0438\u043a\u043d\u043e\u0432\u0435\u043d\u0438\u044e \u0441\u0434\u0432\u0438\u0433\u0430\u044e\u0449\u0435\u0433\u043e \u0443\u0441\u0438\u043b\u0438\u044f \u0438 \u043e\u0431\u0440\u0430\u0437\u043e\u0432\u0430\u043d\u0438\u044e \u043f\u0443\u0437\u044b\u0440\u044c\u043a\u043e\u0432 \u0432 \u0436\u0438\u0434\u043a\u043e\u0441\u0442\u0438, \u0447\u0442\u043e \u043c\u043e\u0436\u0435\u0442 \u043f\u0440\u0438\u0432\u0435\u0441\u0442\u0438 \u043a \u043d\u0435\u0440\u0430\u0432\u043d\u043e\u043c\u0435\u0440\u043d\u043e\u043c\u0443 \u043c\u0430\u0441\u0441\u043e\u043f\u0435\u0440\u0435\u043d\u043e\u0441\u0443 \u043a\u0438\u0441\u043b\u043e\u0440\u043e\u0434\u0430, \u0447\u0442\u043e \u043f\u0440\u0438\u0432\u0435\u0434\u0435\u0442 \u043a \u043d\u0435\u0434\u043e\u0441\u0442\u0430\u0442\u043e\u0447\u043d\u043e\u043c\u0443 \u0441\u043d\u0430\u0431\u0436\u0435\u043d\u0438\u044e \u043d\u0435\u043a\u043e\u0442\u043e\u0440\u044b\u0445 \u043a\u043b\u0435\u0442\u043e\u043a \u043a\u0438\u0441\u043b\u043e\u0440\u043e\u0434\u043e\u043c, \u0447\u0442\u043e \u043f\u043e\u0432\u043b\u0438\u044f\u0435\u0442 \u043d\u0430 \u0440\u043e\u0441\u0442 \u043a\u043b\u0435\u0442\u043e\u043a \u0438 \u043a\u0430\u0447\u0435\u0441\u0442\u0432\u043e \u043f\u0440\u043e\u0434\u0443\u043a\u0446\u0438\u0438.<\/li>\n
    2. \u0421\u043b\u043e\u0436\u043d\u043e\u0441\u0442\u044c \u043e\u0431\u0440\u0430\u0431\u043e\u0442\u043a\u0438 \u0432\u044b\u0441\u043e\u043a\u043e\u0432\u044f\u0437\u043a\u0438\u0445 \u0436\u0438\u0434\u043a\u043e\u0441\u0442\u0435\u0439: \u043f\u0440\u0438 \u0440\u0430\u0431\u043e\u0442\u0435 \u0441 \u0432\u044b\u0441\u043e\u043a\u043e\u0432\u044f\u0437\u043a\u0438\u043c\u0438 \u043a\u0443\u043b\u044c\u0442\u0443\u0440\u0430\u043b\u044c\u043d\u044b\u043c\u0438 \u0436\u0438\u0434\u043a\u043e\u0441\u0442\u044f\u043c\u0438 \u0431\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u044b \u0441 \u043c\u0435\u0448\u0430\u043b\u043a\u043e\u0439 \u043c\u043e\u0433\u0443\u0442 \u0441\u0442\u043e\u043b\u043a\u043d\u0443\u0442\u044c\u0441\u044f \u0441 \u043f\u0440\u043e\u0431\u043b\u0435\u043c\u0430\u043c\u0438, \u0441\u0432\u044f\u0437\u0430\u043d\u043d\u044b\u043c\u0438 \u0441 \u0442\u0440\u0443\u0434\u043d\u044b\u043c \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u043d\u0438\u0435\u043c \u0438 \u0432\u044b\u0441\u043e\u043a\u0438\u043c \u043f\u043e\u0442\u0440\u0435\u0431\u043b\u0435\u043d\u0438\u0435\u043c \u044d\u043d\u0435\u0440\u0433\u0438\u0438.<\/li>\n
    3. \u0418\u0437\u0431\u0435\u0433\u0430\u0439\u0442\u0435 \u0440\u0430\u0437\u0440\u0443\u0448\u0435\u043d\u0438\u044f \u043a\u043b\u0435\u0442\u043e\u043a: \u0434\u043b\u044f \u043d\u0435\u043a\u043e\u0442\u043e\u0440\u044b\u0445 \u0431\u043e\u043b\u0435\u0435 \u0445\u0440\u0443\u043f\u043a\u0438\u0445 \u043a\u043b\u0435\u0442\u043e\u043a \u0441\u0438\u043b\u0430 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u043d\u0438\u044f \u0432 \u0431\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u0435 \u0441 \u043c\u0435\u0448\u0430\u043b\u043a\u043e\u0439 \u043c\u043e\u0436\u0435\u0442 \u043f\u0440\u0438\u0432\u0435\u0441\u0442\u0438 \u043a \u0440\u0430\u0437\u0440\u0443\u0448\u0435\u043d\u0438\u044e \u0438\u043b\u0438 \u043f\u043e\u0432\u0440\u0435\u0436\u0434\u0435\u043d\u0438\u044e \u043a\u043b\u0435\u0442\u043e\u043a, \u0442\u0435\u043c \u0441\u0430\u043c\u044b\u043c \u0432\u043b\u0438\u044f\u044f \u043d\u0430 \u0440\u043e\u0441\u0442 \u0438 \u0432\u044b\u0445\u043e\u0434 \u043a\u043b\u0435\u0442\u043e\u043a.<\/li>\n<\/ol>\n

      \u0411\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u044b \u0441 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u0435\u043c\u044b\u043c \u0440\u0435\u0437\u0435\u0440\u0432\u0443\u0430\u0440\u043e\u043c \u0442\u0430\u043a\u0436\u0435 \u0432 \u043e\u0441\u043d\u043e\u0432\u043d\u043e\u043c \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u0443\u044e\u0442\u0441\u044f \u0432 \u0441\u0443\u0441\u043f\u0435\u043d\u0437\u0438\u043e\u043d\u043d\u043e\u0439 \u043a\u043b\u0435\u0442\u043e\u0447\u043d\u043e\u0439 \u043a\u0443\u043b\u044c\u0442\u0443\u0440\u0435. \u041d\u0438\u0436\u0435 \u043f\u0440\u0438\u0432\u0435\u0434\u0435\u043d\u044b \u043d\u0435\u043a\u043e\u0442\u043e\u0440\u044b\u0435 \u0438\u0437 \u043e\u0441\u043d\u043e\u0432\u043d\u044b\u0445 \u0441\u0446\u0435\u043d\u0430\u0440\u0438\u0435\u0432 \u043f\u0440\u0438\u043c\u0435\u043d\u0435\u043d\u0438\u044f:<\/p>\n

        \n
      1. \u0420\u043e\u0441\u0442 \u043a\u043b\u0435\u0442\u043e\u043a: \u0411\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u044b \u0441 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u0435\u043c\u044b\u043c \u0440\u0435\u0437\u0435\u0440\u0432\u0443\u0430\u0440\u043e\u043c \u0447\u0430\u0441\u0442\u043e \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u0443\u044e\u0442\u0441\u044f \u043d\u0430 \u044d\u0442\u0430\u043f\u0435 \u0440\u043e\u0441\u0442\u0430 \u043a\u043b\u0435\u0442\u043e\u043a \u0432 \u043f\u0440\u043e\u0446\u0435\u0441\u0441\u0435 \u043a\u0443\u043b\u044c\u0442\u0438\u0432\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f \u043a\u043b\u0435\u0442\u043e\u043a. \u0411\u043b\u0430\u0433\u043e\u0434\u0430\u0440\u044f \u0441\u0438\u0441\u0442\u0435\u043c\u0435 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u043d\u0438\u044f \u0438 \u0432\u0435\u043d\u0442\u0438\u043b\u044f\u0446\u0438\u0438 \u043a\u043b\u0435\u0442\u043a\u0438 \u043c\u043e\u0433\u0443\u0442 \u043f\u043e\u043b\u0443\u0447\u0430\u0442\u044c \u0434\u043e\u0441\u0442\u0430\u0442\u043e\u0447\u043d\u043e\u0435 \u043a\u043e\u043b\u0438\u0447\u0435\u0441\u0442\u0432\u043e \u043f\u0438\u0442\u0430\u0442\u0435\u043b\u044c\u043d\u044b\u0445 \u0432\u0435\u0449\u0435\u0441\u0442\u0432 \u0438 \u043a\u0438\u0441\u043b\u043e\u0440\u043e\u0434\u0430, \u0430 \u043a\u043b\u0435\u0442\u043a\u0438 \u043c\u043e\u0433\u0443\u0442 \u043d\u0430\u0445\u043e\u0434\u0438\u0442\u044c\u0441\u044f \u0432 \u0441\u0443\u0441\u043f\u0435\u043d\u0437\u0438\u0438 \u0434\u043b\u044f \u0441\u0442\u0438\u043c\u0443\u043b\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f \u0440\u043e\u0441\u0442\u0430 \u0438 \u0440\u0430\u0437\u043c\u043d\u043e\u0436\u0435\u043d\u0438\u044f \u043a\u043b\u0435\u0442\u043e\u043a.<\/li>\n
      2. \u042d\u043a\u0441\u043f\u0440\u0435\u0441\u0441\u0438\u044f \u0431\u0435\u043b\u043a\u0430: \u0412 \u044d\u043a\u0441\u043f\u0440\u0435\u0441\u0441\u0438\u0438 \u0431\u0435\u043b\u043a\u0430 \u0431\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u044b \u0441 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u044e\u0449\u0438\u043c \u0431\u0430\u043a\u043e\u043c \u043c\u043e\u0433\u0443\u0442 \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u043e\u0432\u0430\u0442\u044c\u0441\u044f \u0432 \u043f\u0440\u043e\u0446\u0435\u0441\u0441\u0430\u0445 \u043a\u0443\u043b\u044c\u0442\u0438\u0432\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f \u043a\u043b\u0435\u0442\u043e\u043a \u0438 \u044d\u043a\u0441\u043f\u0440\u0435\u0441\u0441\u0438\u0438 \u0431\u0435\u043b\u043a\u0430. \u0420\u0435\u0433\u0443\u043b\u0438\u0440\u0443\u044f \u0442\u0430\u043a\u0438\u0435 \u043f\u0430\u0440\u0430\u043c\u0435\u0442\u0440\u044b, \u043a\u0430\u043a \u0441\u043a\u043e\u0440\u043e\u0441\u0442\u044c \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u043d\u0438\u044f, \u0442\u0435\u043c\u043f\u0435\u0440\u0430\u0442\u0443\u0440\u0430 \u0438 \u043f\u0438\u0442\u0430\u0442\u0435\u043b\u044c\u043d\u044b\u0435 \u0432\u0435\u0449\u0435\u0441\u0442\u0432\u0430, \u043c\u043e\u0436\u043d\u043e \u043e\u043f\u0442\u0438\u043c\u0438\u0437\u0438\u0440\u043e\u0432\u0430\u0442\u044c \u0440\u043e\u0441\u0442 \u043a\u043b\u0435\u0442\u043e\u043a \u0438 \u0443\u0440\u043e\u0432\u043d\u0438 \u044d\u043a\u0441\u043f\u0440\u0435\u0441\u0441\u0438\u0438 \u0431\u0435\u043b\u043a\u0430.<\/li>\n
      3. \u041f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u043e \u0432\u0430\u043a\u0446\u0438\u043d: \u0411\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u044b \u0441 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u043d\u0438\u0435\u043c \u0448\u0438\u0440\u043e\u043a\u043e \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u0443\u044e\u0442\u0441\u044f \u0432 \u043f\u0440\u043e\u0446\u0435\u0441\u0441\u0435 \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0430 \u0432\u0430\u043a\u0446\u0438\u043d. \u041e\u043f\u0442\u0438\u043c\u0438\u0437\u0438\u0440\u0443\u044f \u0443\u0441\u043b\u043e\u0432\u0438\u044f \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u043d\u0438\u044f \u0438 \u043a\u0443\u043b\u044c\u0442\u0438\u0432\u0438\u0440\u043e\u0432\u0430\u043d\u0438\u044f, \u043c\u043e\u0436\u043d\u043e \u0443\u043b\u0443\u0447\u0448\u0438\u0442\u044c \u0432\u044b\u0445\u043e\u0434 \u0438 \u043a\u0430\u0447\u0435\u0441\u0442\u0432\u043e \u0432\u0430\u043a\u0446\u0438\u043d, \u0430 \u0442\u0430\u043a\u0436\u0435 \u043e\u0431\u0435\u0441\u043f\u0435\u0447\u0438\u0442\u044c \u0441\u0442\u0430\u0431\u0438\u043b\u044c\u043d\u043e\u0441\u0442\u044c \u0430\u043a\u0442\u0438\u0432\u043d\u044b\u0445 \u0438\u043d\u0433\u0440\u0435\u0434\u0438\u0435\u043d\u0442\u043e\u0432 \u0432 \u0432\u0430\u043a\u0446\u0438\u043d\u0430\u0445.<\/li>\n
      4. \u041f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u043e \u0430\u043d\u0442\u0438\u0431\u0438\u043e\u0442\u0438\u043a\u043e\u0432: \u0412 \u043f\u0440\u043e\u0446\u0435\u0441\u0441\u0435 \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0430 \u0430\u043d\u0442\u0438\u0431\u0438\u043e\u0442\u0438\u043a\u043e\u0432 \u0431\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u044b \u0441 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u044e\u0449\u0438\u043c \u0443\u0441\u0442\u0440\u043e\u0439\u0441\u0442\u0432\u043e\u043c \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u0443\u044e\u0442\u0441\u044f \u0434\u043b\u044f \u0432\u044b\u0440\u0430\u0449\u0438\u0432\u0430\u043d\u0438\u044f \u0438 \u0444\u0435\u0440\u043c\u0435\u043d\u0442\u0430\u0446\u0438\u0438 \u043c\u0438\u043a\u0440\u043e\u043e\u0440\u0433\u0430\u043d\u0438\u0437\u043c\u043e\u0432. \u041f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u044f \u0438 \u043a\u043e\u043d\u0442\u0440\u043e\u043b\u0438\u0440\u0443\u044f \u0442\u0435\u043c\u043f\u0435\u0440\u0430\u0442\u0443\u0440\u0443 \u0440\u0435\u0430\u043a\u0446\u0438\u0438, \u0437\u043d\u0430\u0447\u0435\u043d\u0438\u0435 pH \u0438 \u0434\u0440\u0443\u0433\u0438\u0435 \u0443\u0441\u043b\u043e\u0432\u0438\u044f, \u043c\u043e\u0436\u043d\u043e \u0443\u0432\u0435\u043b\u0438\u0447\u0438\u0442\u044c \u0441\u043a\u043e\u0440\u043e\u0441\u0442\u044c \u0440\u043e\u0441\u0442\u0430 \u0448\u0442\u0430\u043c\u043c\u043e\u0432 \u043c\u0438\u043a\u0440\u043e\u043e\u0440\u0433\u0430\u043d\u0438\u0437\u043c\u043e\u0432 \u0438 \u0432\u044b\u0445\u043e\u0434 \u0430\u043d\u0442\u0438\u0431\u0438\u043e\u0442\u0438\u043a\u043e\u0432.<\/li>\n
      5. \u041f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u043e \u043a\u043e\u0441\u043c\u0435\u0442\u0438\u0447\u0435\u0441\u043a\u043e\u0439 \u043f\u0440\u043e\u0434\u0443\u043a\u0446\u0438\u0438: \u0411\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u044b \u0441 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u044e\u0449\u0438\u043c \u0431\u0430\u043a\u043e\u043c \u0438\u0441\u043f\u043e\u043b\u044c\u0437\u0443\u044e\u0442\u0441\u044f \u0434\u043b\u044f \u044d\u0442\u0430\u043f\u043e\u0432 \u0441\u043c\u0435\u0448\u0438\u0432\u0430\u043d\u0438\u044f \u0438 \u0440\u0435\u0430\u043a\u0446\u0438\u0438 \u0432 \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0435 \u043a\u043e\u0441\u043c\u0435\u0442\u0438\u0447\u0435\u0441\u043a\u043e\u0439 \u043f\u0440\u043e\u0434\u0443\u043a\u0446\u0438\u0438. \u041f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u044f \u0438 \u043a\u043e\u043d\u0442\u0440\u043e\u043b\u0438\u0440\u0443\u044f \u0443\u0441\u043b\u043e\u0432\u0438\u044f \u0440\u0435\u0430\u043a\u0446\u0438\u0438, \u043c\u043e\u0436\u043d\u043e \u0433\u0430\u0440\u0430\u043d\u0442\u0438\u0440\u043e\u0432\u0430\u0442\u044c, \u0447\u0442\u043e \u0441\u044b\u0440\u044c\u0435 \u043f\u043e\u043b\u043d\u043e\u0441\u0442\u044c\u044e \u0441\u043c\u0435\u0448\u0430\u043d\u043e, \u0430 \u043a\u0430\u0447\u0435\u0441\u0442\u0432\u043e \u043f\u0440\u043e\u0434\u0443\u043a\u0442\u0430 \u0443\u043b\u0443\u0447\u0448\u0435\u043d\u043e.<\/li>\n<\/ol>\n

        \u041f\u0440\u0438\u043c\u0435\u043d\u0435\u043d\u0438\u0435 \u0431\u0438\u043e\u0440\u0435\u0430\u043a\u0442\u043e\u0440\u043e\u0432 \u0441 \u043f\u0435\u0440\u0435\u043c\u0435\u0448\u0438\u0432\u0430\u044e\u0449\u0438\u043c \u0443\u0441\u0442\u0440\u043e\u0439\u0441\u0442\u0432\u043e\u043c \u0432 \u0441\u0443\u0441\u043f\u0435\u043d\u0437\u0438\u043e\u043d\u043d\u043e\u0439 \u043a\u0443\u043b\u044c\u0442\u0443\u0440\u0435 \u043a\u043b\u0435\u0442\u043e\u043a \u043c\u043e\u0436\u0435\u0442 \u043e\u0431\u0435\u0441\u043f\u0435\u0447\u0438\u0442\u044c \u043a\u043b\u0435\u0442\u043a\u0430\u043c \u0445\u043e\u0440\u043e\u0448\u0443\u044e \u0441\u0440\u0435\u0434\u0443 \u0440\u043e\u0441\u0442\u0430 \u0438 \u043e\u043f\u0442\u0438\u043c\u0438\u0437\u0438\u0440\u043e\u0432\u0430\u043d\u043d\u044b\u0435 \u0443\u0441\u043b\u043e\u0432\u0438\u044f \u0440\u0435\u0430\u043a\u0446\u0438\u0438 \u0434\u043b\u044f \u043f\u043e\u0432\u044b\u0448\u0435\u043d\u0438\u044f \u044d\u0444\u0444\u0435\u043a\u0442\u0438\u0432\u043d\u043e\u0441\u0442\u0438 \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0430 \u0438 \u043a\u0430\u0447\u0435\u0441\u0442\u0432\u0430 \u043f\u0440\u043e\u0434\u0443\u043a\u0446\u0438\u0438.<\/p>","protected":false},"excerpt":{"rendered":"

        The stirred tank bioreactor is one of the most common suspended cell culture bioreactors. It keeps the cells in the culture in a suspended state by mechanical stirring and provides sufficient oxygen and nutrients for cell growth. The structure of the stirred tank bioreactor is relatively simple and easy to operate and maintain. However, the […]<\/p>\n","protected":false},"author":1,"featured_media":3873,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[],"class_list":["post-3871","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bailun"],"_links":{"self":[{"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/posts\/3871","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/comments?post=3871"}],"version-history":[{"count":3,"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/posts\/3871\/revisions"}],"predecessor-version":[{"id":5459,"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/posts\/3871\/revisions\/5459"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/media\/3873"}],"wp:attachment":[{"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/media?parent=3871"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/categories?post=3871"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/fermentorchina.com\/ru\/wp-json\/wp\/v2\/tags?post=3871"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}