Biorreactor de vidrio con agitación mecánica 5L 3Union (externo)

What is the 5L 3Union Mechanical Agitation Glass Bioreactor (Off-site)?

At its core, the Biorreactor de vidrio con agitación mecánica 5L 3Union (externo) is a next-generation lab-scale fermentation system. It is designed meticulously with 1 tank (5-liter glass), strong mechanical stirring, and stainless steel 316L connections and hence can fit in any application of biotechnology.

Its modular design, intelligent control via Siemens PLC, and compact footprint allow researchers to run highly controlled fermentations, whether you’re growing bacteria, yeast, plant cells, or animal cells.

The term “off-site” comes up because it can be sterilized in an autoclave, which also makes it much easier to clean up and which spares you the complicated SIP set-ups, making it perfect in an academic laboratory, when doing piloting or when making very low quantities of an item in production.

Who Is It for?

• Biotech companies needing controlled fermentation in R&D
• Universities and research labs studying microbial or mammalian cells
• Pharmaceutical facilities performing vaccine or enzyme production
• Food & bioengineering institutions conducting strain optimization

Why Choose This Bioreactor?

Have you ever had the experience of unreliable results, hard to setup, or lack of control of your fermentation?

El Biorreactor de vidrio con agitación mecánica 5L 3Union (externo) addresses these issues head-on:

• Simple setup, yet powerful functionality
• Ability to control pH, DO, temperature, and agitation among others to a full extent
• Reliable mixing with precision-built mechanical stirrers
• Real-time monitoring and data export for analysis
• Compact, elegant design, ideal for any lab bench

Now what exactly would you get with this new solution? Let’s explore the system in detail.

Descripción del fermentador

El Biorreactor de vidrio con agitación mecánica 5L 3Union (externo) fermentation system integrates cutting-edge technology with simplicity. It has a glass tank, 3Union mechanical top drive and high quality components that can support the batch, fed-batch or even sensitive cell culture.

Both fermentation tanks have the support of a front mounted control box with a build in control system including four built in peristaltic pumps to dose acid, base, deformer and nutrients creating automation and ease of use with each run.

With its smart, touchscreen controller, sturdy Swiss-made sensors and many other features, this system conveys prudent engineering designed to surpass the requirements of the modern bioprocessing challenge.

Technical Specifications

A closer inspection of detailed specifications of this industry-grade fermentation system is as follows.

Volume Specifications

This model has a total volume of 5 liters which is ideal when doing medium scale experiments and trials.

Tank Design

Full visibility is achieved because it is made out of borosilicate glass and that the stainless steel used enables a sterile and durable setup because it is resistant to corrosion.

Sistema de agitación

• Mechanical drive (GJ type – top-mounted)
• Includes:

1. 3 six-blade paddles
2. 1 foam breaker
3. Height adjustable paddles

• Stir speed:
• Mechanical: 50–1000 rpm
• Magnetic (optional): 50–500 rpm

This mixing offers a soft, but highly efficient mixing and guarantees transfer of oxygen and homogeneity even in the highly delicate cultures.

Sterilization Method

• Off-site sterilization via autoclave

(No in-site sterilization hardware needed)

Is your lab equipped with an autoclave? In that case, this is the ideal because they can sterilize the vessel safely and reliably, without an in-built steam generator.

Gas Control System

• Rotor meter regulated airflow
• Air flow range: 1–2 vvm
• Compatible with O₂/N₂/CO₂ configurations for various fermentation environments

Feeding System

Four peristaltic pumps for:

• Acid
• Base
• Antifoam
• Culture media
• Positioned on front control box
• Programmable feeding control via PLC interface

No more manual dosing- automate everything on your feeding process.

Temperature Control

• Electric heating system
• Water-jacket-based control
• Temperature range: 5°C to 70°C
• Fully programmable

Maintain optimal conditions for a broad range of organisms.

pH & DO Control

• pH range: 2.00–12.00 ±0.1
• DO range: 0–150 ±3%, resolution 0.1%
• Sensors: High-end probes from Hamilton/Mettler (Switzerland)
• Automatic acid/base and oxygen modulation

Worried about pH drift or oxygen depletion? Not with this system. Adjustments are automatic and real-time.

Sampling Port

• Manual sampling for easy access without contamination
• Extract samples for offline analysis while maintaining sterile integrity

Siemens PLC Control, Intelligence at Your Fingertips

The built-in Siemens PLC system has a programmable internal controller to control the entire fermentation run with a 10-inch or 15-inch touch screen. Monitor, manage, and log every parameter with ease.

Control Features:

• Stirring speed
• pH and DO monitoring
• Temperature regulation
• Feeding rate
• control de gases
• Alarm alerts
• Data export and trending
• Language support: EN, RU, CN

The result? You stay informed and in control at every stage.

Optional Add-Ons, Customize Your System

Need extra functionality? Add optional modules to expand your setup:

• Remote control functionality
• Mass Flow Meter (automated gas control)
• O₂/N₂/CO₂ gas control modules
• Exhaust gas (CO₂) analysis
• Level and turbidity (OD) sensors
• ORP (redox potential) sensor
• Biomass monitoring sensors
• Weight-based feed control
• Live data printing
• Siemens industrial-grade upgrades

Application Areas

El Biorreactor de vidrio con agitación mecánica 5L 3Union (externo) is ideal for:

Human and Animal Vaccine Production

• Hepatitis B, Influenza, Rabies, Foot-and-mouth disease, Tetanus, Meningitis, etc.

Microbial & Cell Culture

• CHO and HEK293 cells
• Yeast (S. cerevisiae, P. pastoris)
• Bacterial strains (E. coli, Bacillus subtilis)
• Algae and plant cells

Biotech & Pharmaceutical R&D

• Antibiotic screening
• Enzyme and protein production
• Nutraceutical development

Academic Research & Teaching Labs

• Genetic engineering
• Bioprocess optimization
• Cell line testing and development

Searching a reliable system, which incorporates various applications within a single device? This is your answer.

Why It Stands Out?

Still wondering what sets this bioreactor apart?

Here are just a few standout advantages:

✅ Visibilidad: Transparent glass vessel for real-time observation

✅ Accuracy: High-precision pH/DO sensors

✅ Automation: 4-pump peristaltic feeding and foam control

✅ Flexibility: Compatible with batch and fed-batch operations

✅ Integration: Connect to your PC or control software

✅ Compact Design: Minimal footprint, maximum performance

Concluding Remarks

If you’re looking for a lab-scale bioreactor that’s affordable, powerful, and precise, then the Biorreactor de vidrio con agitación mecánica 5L 3Union (externo) is the tool for the job. Whether you’re culturing microbes, producing recombinant proteins, or developing the next vaccine breakthrough, this system will help you achieve consistent and scalable results.

So, are you ready to transform your fermentation workflow? Explore the Biorreactor de vidrio con agitación mecánica 5L 3Union (externo) on BaiLun Bio and request a quote tailored to your lab’s unique needs.

Frequently Asked Questions (FAQs)

Q1: How do I sterilize the vessel?

Sterilization is performed off-site using an autoclave. Glass vessel and stainless steel lid are easily distributed to be cleaned and sterilized.

Q2: Is it possible to upgrade to SIP or a bigger volume in the future?

Yes! BaiLun Bio also has all varieties of scale-up purposes that include a complete line of SIP productions and more extensive volumes.

Q3: Is it suitable for sensitive cell lines?

Absolutely. Mechanical agitation system can be regulated and is conducive to gentle stirring and thus ideal on CHO, HEK293 and other mammalian types.

Q4: Is the software easy to use?

Yes. The Siemens PLC front end is user friendly and the multilingual touch screen is totally visible and allows control of parameters.

Q5: Will I be able to design the ports or add some specific sensors?

Yes. Several optional systems may be provided by the system e.g., ORP sensors, OD probes and biomass sensors, etc