Meet the Six union of the stainless steel fermenter (100L)
Want to enhance the fermentation phase in your laboratory using a bioreactor with huge capacity that can be controlled precisely? The Six union of the stainless steel fermenter (100L) offers everything you need, modularity, robustness, and automation, packed into an industrial-strength chamber.
It is manufactured out of high grade stainless steel, employing Siemens automation PLC and designed to be used in research and pilot scale, such fermenter will be a prime performer and provide a reliable operation.
Curious how “six‑union” modules can streamline your workflows? Curious why labs across the globe are turning 100 L into their powerhouse? Let’s dive in!
What Does “Six Union” Really Mean?
- Six combined modules port:Multiple sampling, gas, nutrient and sensor connection into one aesthetically designed top-head.
- Modular head plate configuration:Six Union allows your head plate to be configured with ports specified to suit your process spargers, sampling, antifoam, sensors, feeding, harvest, etc.
- Large scale at a small size:The 100 L working volume is like a pilot-scale system but without over dominating laboratory space.
Why Choose the Six Union of The Stainless Steel Fermenter (100L)?
1. Versatile Port Configuration
With so many ports confidently available at the head as a union-style union, you can design your ferment process comfortably, with no penalties to sterilization or performance: You can device in filters, harvest lines, probes, or even forthcoming update to your fanciful, all without interruptions to sterilization or activity.
2. Proven Sterility & Clean ability
With SIP (sterilize-in-place) and CIP (clean-in-place), the system lets you maintain aseptic conditions without disassembly. ZERO dead zones, polished SS316 interior (0.4–0.6 μm), and sanitary bottom valves showcase optimized hygienic design.
3. Automation at the Helm
It has a Siemens-driven PLC and a color touchscreen (10 or 15 inches), which allows the users to indulge in a complete control of the stirring, the temperature, the pH, the DO, the gas flow and the pump dosing. Want data logs, trend graphs, or instant alarms? It’s all built-in.
4. Dual Drive Options
With 3 six-blade impellers, and either a stable agitation, 50rpm to 1000rpm mechanical, or a magnetic drive, 50rpm to 400rpm, you can select the top-drive mechanical stirring, or a bottom magnetic drive; or a combination of either one or both.
Technical Specifications
| PARAMETER | DESCRIPTION |
| Total volume | 5 L, 10 L, 15 L, 20 L, 30 L, 50 L, 100 L, 200 L, 500 L, 1000 L, 5KL, 50KL, 200KL etc. |
| Working volume | 30 % – 70 % of total capacity |
| Tank | Jacketed SS316/SS304; vessel pressure 0.3 MPa, jacket 0.35 MPa; 0.4–0.6 µm polished; height-to-diameter 1:2.2–2.5 |
| Stirring system | Mechanical top/bottom or magnetic top drive; 3 adjustable six-blade paddles |
| Stir speed | 50–1000 rpm (mechanical) / 50–400 rpm (magnetic) |
| Sterilization | Fully automatic SIP controller |
| Cleaning | CIP via onboard spray ball |
| Gas control | Rotor meter-controlled airflow (1 vvm standard) |
| Feeding | Automatic peristaltic pump nutrient dosing |
| Anti-foam control | Automatic via peristaltic dosing |
| Temperature control | Auto-controlled; 5–70 °C (electric/water) |
| pH control | 2.00–12.00 ± 0.1; acid/base dosing; Hamilton/Mettler sensor |
| DO control | 0–150 % ± 3 % (0.1 % precision); Hamilton/Mettler sensor; coupled with agitation and oxygen |
| Sampling | Manual sterilizable sample valve |
| Harvest | Zero-dead-zone bottom valve (Gemu/Bürkert, Germany), sterilizable, manual or auto |
| Lid lift system | 30–500 L: hydraulic lid lift; 700–1000 L: human access port |
| Pressure | Auto pressure control via BD Germany transmitter |
| Exhaust | Condenser + diaphragm / proportional control valve |
| Control parameters | Siemens PLC with 10″/15″ touchscreen; stirring, pH, DO, temp, feeding, alarm, gas control, data record, multilingual (RU/EN/CN), customizable |
| Optional configuration | Remote control, mass flow meter, O₂/N₂/CO₂ gas mix (up to 2 vvm), CO₂ exhaust analysis, level, turbidity, ORP, weight, live cell sensors, printing, Siemens HMI, double seal, 4-valve feeding group, bottom auto-discharge, ASME/CSA, batch, seed modes |
Engineered to Perform
✅ Stainless Steel Excellence
Made of high quality SS316L/SS304 that has mirror polish finish- this one is hygienic stainless steel at its best. No deader zones just clean and shiny welds.
✅ Six-Way Head Plate
Mount six sterilizable unions that are individually sterilizable which are used most commonly in sparger, antifoam, nutrient, sampling, gas outlet, probes among others. Flexible, modular, and ready for complex fermentation processes.
✅ Clean SIP/CIP Design
SIP steam-jacket sterilization can keep your system aseptic, without the arms-on trouble, as well as CIP spray ball cleaning. Automated sequences bring consistency to every run.
✅ Input-Driven Automation
Peristaltic pumps respond in real-time to the sensor input, e.g., when adding a base to correct pH, antifoam, nutrients, or adjusting the gas and the rotor meters against one another.
✅ Sensor Network
Featuring Hamilton/Mettler sensors for DO and pH, German pressure sensors, and optional additions (ORP, turbidity, biomass, CO₂), data-driven decisions, always.
✅ Powerful Control Hub
Siemens PLC ensures your fermentation is automated in more than one language, and there is logging, alarms, trend monitoring, configuration profiles and export to LIMS or Excel and it does not require additional software.
Who is This Six Union of the Stainless Steel Fermenter (100L) for?
- Biotech developers producing vaccines, proteins, or viral vectors
- Microbial fermentation labs scaling screening to pilot
- Academic researchers exploring parallel fermentation setups
- Starter kits for CDMOs needing versatile production-ready equipment
- Process engineers implementing fed-batch or continuous fermentation workflows
Application Scenarios
| Use Case | Why You Would Choose This Fermenter? |
| Vaccine production | Standardized, scalable 100 L runs with top-quality monitoring and sterility |
| Protein expression | Dual agitation and DO sensors create homogenous mixing and high yield—without compromise |
| Biopharma R&D scaling | Mirror production systems with pilot and lab units. Reproducible workflows built in |
| Process validation | Six unions enable multi-strategy sampling and parallel cultivation setups |
| Dual colonization studies | Configure duplicate sensors/probes to assess batch vs fed-batch strategies in one run |
What Sets This Apart?
✅ Six-port union flexibility – Add or remove ports easily without compromising sterility
✅ Premium hardware – German valves, Swiss sensors, polished SS316 interior
✅ Automated, intelligent control – Siemens PLC tracks every parameter, from gas flow to pump dosing
✅ Global-ready UI – Intuitive interface in English, Chinese, or Russian
✅ Expandable platform – Add CO₂, turbidity, biomass sensors, or scale up to 200–500 L versions
Real-World Lab Integration
Have you ever wanted to compare two conditions (e.g. pH 6.8 and 7.2) and carry them out simultaneously at the same temperature and stirring rate? The Six union of the stainless steel fermenter (100L) lets you do just that.
Perfect for:
- Multi-run method validation
- Batch versus fed-batch comparison
- Seed expansion and production in the same unit
- Continuous fermentation with regular sampling
- Sterile transfer-ready setups using auto bottom valves
Conclusion
From its polished SS316 construction and intelligent six-union top plate to Siemens automation and modular expandability, the Six union of the stainless steel fermenter (100L) is a fermentation game changer. It aligns your future-ready requirement- accurate sampling processes to the huge-scale pilot operation with a sound design and complete process management.
Don t you think that your laboratory could use a fermenter that can be stretched enough to operate both at R&D and production levels? Order a demonstration or receive a custom quote today, or come see us to see add-ons such as CO2 analysis, remote access tools, or sensor packages.
Frequently Asked Questions (FAQ)
Q: What is “six union” all about?
Each fermenter head carries six individually configurable ports (unions). Wish to retrospectively add an ORP sensor or custom sampling port? Simply swap a blank with a sensor-equipped union.
Q: Is this tank easy to clean and sterilize?
Absolutely, 0.4–0.6 µm internal polish prevents biomass sticking. Sterilization is made easier with automated SIP and CIP cycles and this ensures that each run receives an undefiled tank.
Q: What type of agitation drive is supported?
Choose between top mechanical drive (rigid torque) or magnetic bottom drive (sealed, no penetration). Both use triple six-blade impellers.
Q: How do I extract data?
The Siemens PLC supports USB, RS232, and Ethernet. Directly transfer pH, DO, temperature and setpoint trends to your LIMS or Excel.
Q: Can this ferment complex mammalian cell cultures?
Yes, including CHO, HEK293, or Vero. It is cell culture ready (2) with temperature range 5 70 C, exact DO/pH control, automatic foaming control and sterile harvest.
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