Cellislet® fiber cell disks is a solid support matrix for mammalian, animal and insect cells growth, mainly for secreted products (such as recombinant proteins and viruses). It is made of high purity polyester fiber which is suitable for cell adherent growth, and the material used meets the requirements of USP<88> plastic classification VI. The effective surface area of each gram of carrier is about 1800~2200cm2, which has highly efficient utilization of surface area and three-dimensional spatial structure, and it can maintain high-density growth of perfusion culture for a long period of time, avoiding the risk of clogging; and reducing the need of cell filtration from the final product. and reduce the need for cell filtration from the final product.
Cell Disks Characteristics:
- Low pressure loss in the bed through the culture basket reduces variability and maintains cell activity in the bed;
- High surface volume ratio increases biomass and maintains high bioreactor throughput;
- Embedded cells are protected from eddy currents and avoid damage due to shear forces from stirring paddles and deep aeration bubbles;
- High nutrient and oxygen delivery rates compared to traditional microcarrier systems;
- Cellislet® tablets are manufactured and tested under strict quality guidelines;
- Composed of polyester nonwoven fibers that meet USP Class VI requirements.
应用:
- Hybridomas: DA4-4, 123A, 127A, GAMMA, 67-9-B, etc;
- Adherent cells: 3T3, COS, human osteosarcoma cells, MRC-5, BHK, VERO, CHO, rCHO-tPA, rCHO – Hepatitis B Surface Antigen, HEK 293, rHEK 293, rC127 – Hepatitis B Surface Antigen, normal human fibroblasts, mesenchymal stromal cells, hepatocytes, etc;
- Insect cells: Tn-368, SF9, rSF9, Hi-5, etc.
| Testing items | Indicator | Citation standard |
| Bioburden | ≤30cfu/g | Chinese Pharmacopoeia, 2020 Edition, Part IV, General Provisions 1105 |
| Cytotoxicity | 0 | Biological evaluation of medical devices Part 5: In vitro cytotoxicity test |
| Endotoxin | ≤0.5EU/ml | Chinese Pharmacopoeia, 2020 edition, four parts, General rule 1143 |
| Number of layers | 3~7 layers | |
| Appearance | White color, no impurities |
Product | Item No. | Packing specification |
Cellislet® fiber cell disks | MC2001A | 1000g/pack |
Cellislet®fiber cell disks (alkali washed) | MC2001B | 1000g/package |
Cellislet® fiber cell disks | MC2002A | 500g/package |
Cellislet® fiber cell disks (alkali washed) | MC2002B | 500g/package |
Cellislet® fiber cell disks (MC2001A) generally follows the following procedure when used:
- Soak overnight in 0.25-0.5 mol/lL sodium hydroxide.
- Rinse well in pure water transfer to PBS solution and soak and wash 2-3 times.
- Load into reactor and sterilize for 40 minutes, loading volume 30-40g/L calculated.
- After cooling, withdraw the PBS and infuse the cell culture for aseptic test for 48 hours.
- Withdraw the sterile test medium, introduce fresh medium and access the cells to be cultured.
- Perform normal culture state.