Multi-stage Glass Bioreactor(off-site sterilization)

Why Is the Multi-stage Glass Bioreactor Unique?

The modern climate of high intensity of research and bio production is demanding on the team, requiring an easy to sterilize and operate system that is yet capable of flexibility to suit most fermentation requirements. The Multi-stage Glass Bioreactor delivers all of this and more.

Imagine having the power to precisely monitor and control critical parameters like pH, dissolved oxygen (DO), temperature, and stirring speed, all displayed in real time on a 10 or 15-inch color touch screen. It is meant to give researchers and technicians instant information and complete process control.

Why Multi-stage Glass Bioreactor is an Laboratory Favorite Bioreactor?

• Off-site sterilization (autoclave) for flexible cleaning schedules.
• A clear glass container in case one wants to see the process of fermentation.
• Siemens PLC system of intelligent control and data management.
• Several peristaltic pumps to automatically add acid, base, antifoam and nutrients.
• Optional set ups to suit your individual research requirements.

Does not it sound like the solution, which has been sought by your team?

Multi-stage Glass Bioreactor Technical Parameters: Accuracy in All the Parts Fermented

Behind the scenes of Multi-stage Glass Bioreactor made up of perfectly planned technical parameters, is one of the very potent instruments:

Parameter	Description
Total volume	0.2L,0.5L,1L,2L,3L,5L,10L,20L,30L,40L etc.
Working volume	50%~70%
Tank	Single glass vessel with stainless steel bottom. Lid made by stainless steel 316L with ports, condenser on the cover
Stirring system	Mechanical drive(GJ type) or Magnetic drive(GC type, bottom/top drive), 3 six-blade paddle and 1 foam breaker, each paddle height adjustable. Stir speed: 50~1000rpm/50-500rpm
Sterilization	Off-site sterilization (Autoclave)
Gas control	Control by rotor meter, air flow 1-2vvm.
Feeding	Automatically feeding by peristaltic pump
Anti-foam control	Automatically add antifoamer by peristaltic pump
Temperature control	Auto-control, electric heating range water source add 5℃ to 70℃
PH control	PH 2.00-12.00±0.1, auto-control adding acid/base by peristaltic pump, pH sensor from Hamilton/Mettler, Switzerland.
DO control	0-150±3% displaying precision 0.1%, DO sensor from Hamilton/Mettler, Switzerland. DO coupling with agitation, oxygen.
Sampling	Manual sampling
Control parameters	10 or 15 inch touch screen , Siemens PLC with control parameters (stirring, pH, DO, temperature, feeding, alarm, gas etc.), data analysis, data record, data output, language(RU/EN/CN) and other customized functions
Optional configuration	Remote control, Mass flow meter(auto control gas), O2/N2/CO2, gas(CO2) exhaust analysis, Level sensor, Turbidity(OD) sensor, ORP sensor, weight system, Live cell sensor(Biomass), Printing, Siemens touch screen, etc.,

User-Friendliness and Adaptability

The Multi-stage Glass Bioreactor’s backbone is the BLBIO GJ series system, which is explicitly dedicated to fulfill a wide variety of fermentation modes:

• Batch fermentation
• Fed-batch fermentation
• More and animal cell culture

Single glass vessel plus stainless steel bottom base and lid makes the bioreactor durable, and vessels, at the same time, let you see what is happening with the fermentation process. And isn’t there a time you have wished you knew everything that was being done, and could react to it? That is possible now.

Simultaneously, the upper-driven direct-coupling mechanical stirring system maintains mixing both consistent and stable, and the small overall structure also saves considerable laboratory space.

Why Off-site Sterilization?

Have you ever wondered why off-site sterilization is Important? It is possible using an autoclave external system:

• Processing can be done in parallel on more than one vessel and minimize downtimes.
• There are simplified in-place cleaning systems to ease maintenance.
• It aids better safety particularly in dealing with delicate or risk cultures.

To a lot of research labs and pilot-scale, this is a feature of not only efficiency but of peace of mind.

High-Tech Control to The Last Confidence

With its integrated Siemens PLC system and large color touch screen, the Multi-stage Glass Bioreactor brings process data to your fingertips:

• The stirring speeds are variable between 50 and 1000rpm or 50-500rpm When this is monitored.
• Maintain your set pH levels with Swiss made pH sensors.
• Monitor the levels of DO properly.
• Pump out to cause automation of feeding and addition of antifoam.
• Export, import and analyze data easily.

Wouldn‚t you like it simpler to innovate with an equipment as your intelligent partner?

Applications: Aid in Transformation of a field

The versatility of this bioreactor can be used in endless applications including:

• Production of different animal vaccines:Avian influenza, Rabies, Mink vaccine, Tetanus, DTP and others.
• Human vaccines:Hepatitis B, Pneumonia IV, Meningitis and so on.
• Bacteria, yeast and fungi:microorganism culture.
• Mammalian cell line:CHO, 293 and others.
• Use plant cells and algae:as food, pharmaceutical and nutraceutical.
• Enzyme production: protein both manufacturing and production, additive development, and antibiotics.

Regardless of the nature of your lab, whether basic research, product development or pilot production, this system will comply with your working process.

Benefits of imagination

• Assist you in getting the same results each time?
• Make scale up less complicated between lab bench and pilot plant?
• Are automatic logs going to reduce the manual data recording?
• Give immediate visual account of culture growth?
• Allow a variety of fermentation schemes, batch, fed-batch and continuous?

With the Multi-stage Glass Bioreactor, these are more than possibilities, they’re built-in advantages.

Optional Setups: Set Your Research Configuration

Each process is peculiar. In order to be compatible with your requirements, this system may be customized with:

• State of art gas mixing and monitoring equipment.
• Real time growth monitoring by turbidity and biomass sensors.
• Redox potential ORP sensors.
• Weight systems of exact feed regulation.
• Compliance of batch records to be printed.

Why be satisfied with generic when you can produce the ideal choice to fulfill your project?

Scale-Size Precision of an Industrial Design in A Lab-Friendly Design

That combination of strong stainless steel and transparent glass is not by chance. It ensures:

• Very good corrosion resistant.
• Stuff behind-the-scene.
• Transparency through which it is easy to troubleshoot and optimize.

And with lots of holes on the stainless steel lid, you can add sensors, sample lines, and feed inlets without encountering the crowd in the working area.

IDT from Data to Decisions

Information does not consist of only numbers. It’s insight.

• The PLC based system within the bioreactor converts unprocessed data to useful data:
• Chart time wise changes.
• Identify reasons of variability.
• Export your data to analysis applications smoothly.

Won’t it help to go through complete fermentation runs without flipping through notes written by hand?

Small, Stackable and Expandable

Are there cases when you have to execute many processes? Or compare conditions on side by side basis?

Thanks to its modular design and range of vessel sizes (from 0.2L to 40L), the Multi-stage Glass Bioreactor makes this possible without overwhelming your lab space.

Current Scientists in Mind

User friendly is as important as fast. Important operator-oriented functionality is:

• Front mounted peristaltic pumps to make rapid adjustments to.
• Big touchscreen having simple interface.
• Various options to make the vessels flexible to meet your demands of media and culture.
• Elaborate tools are supposed to make your work easier and not harder.

Why Choose the Multiple Glass Bioreactor (off-site sterilization)?

• Enables a wide variety of applications: microbial, cell, enzyme and vaccine productions.
• Off-site sterilizing that is flexible as to maximize availability and safety.
• Clear container to have direct eye control.
• Able to tightly automate all-important parameters.
• Customizable to suit your research objectives and modular.
• Industrial quality data control based on reliable Siemens PLC.

Food Engineering and Life Sciences: The Future of Fermentation in Your Hands

Each amazing finding begins with a little bit. With the Multi-stage Glass Bioreactor, you have the technology, precision, and flexibility to bring ideas from the bench to the pilot scale, and beyond.

Do you want to make the research of today the breakthrough of tomorrow? We are at your service at BaiLun Bio. Together we can make the future.