Introducing the Three‑stage multi‑stage fermenter 10L‑50L‑100L

Are you willing to enhance your microbial or cell culture-related potential? Here comes the Three-stage multi-stage fermenter 10L-50L-100L, the game-changing bioreactor, carefully hand-made to expand laboratories, develop processes, and perform pilot runs up through tiny industrial scales.

When you are working to develop vaccines or grow mammalian or microbial cell, producing enzymes or pharmaceuticals, this flexible, user-friendly system allows your research or production to be precise, safe and scalable.

Why go multi-stage?

• Flexibility, meet consistency:

Imagine three vessels that would lock into each other seamlessly. Once such vessels could work in parallel or in series. Launch your inoculum at 10 L stage, step up to 50 L, and produce polishing at 100 L. Or run them in tandem to run many conditions concurrently.

• Robust process control equals reproducible results:
  Siemens PLC 10 touch interface coordinates your pH, DO, temperature, stirring and gas flow to ensure that they all run in harmony. Data logging, alarms, set point tracking, everything becomes hands off once dialed-in.
  
• Clean and sterile every run:

SIP (Sterilize-In-Place) and CIP (Clean-In-Place) ensure aseptic conditions without dismantling. The valves, pipelines, heads and sampling systems are completely sterile and the cleanup process is easier than ever before.

Wondering why this is the most recommended fermenter of R&D or pilot teams globally? Let’s dive deeper.

Key Highlights & Benefits

1. Scalable, Modular Design

• Available in 10 L, 50 L, and 100 L stages, but also offered in smaller (5–30 L) and larger (200 L, 500 L, even 1000 L+) configurations.
• They can easily be used individually, or latched together in a continuous chain, so hooking or unhooking many is easy.
• It is lab-friendly due of its small size, as well as floor- or bench-mount configurations. Need centralized control? Merging of several units to a single control cabinet or PC interface.

2. Premium Stainless Steel Construction

• SS316L body does not have dead areas; polished to 0.4 0.6m, ensuring little cleaning inconvenience.
• Smooth welds and hygienic ones, and manhole lids, ports, and glass are all constructed under strong safety standards.
• The jackets and vessels up to 0.3 to 0.35 0 3 9, and up to 2.5 to 1 height to diameter ratios–jackets and vessels–give superior temperature stability.

3. Efficient Mixing, Aeration, & Gas Control

• Three adjustable six‑blade paddles powered by mechanical or magnetic drives (top or bottom).
• Stirring speeds from 50–1000 rpm (mechanical) or 50–400 rpm (mag drive).
• Rotameters to 1 vvm or optional 2 vvm with mass flow control–and built-in spargers that uniformly delivers oxygen.

4. Automated Process Management

• pH (2–12 ± 0.1), DO (0–150 % ± 3 %), temperature (5–70 °C electrical/water heating), foam control, feeding, and harvesting, all automated via Siemens S7 PLC.
• Sensor-grade components (Hamilton/Mettler for DO/pH; German pressure transducers) maximize repeatability and accuracy.
• 10″ or 15″ touchscreens with multi-language support (EN/CN/RU), configurable alarms, data output, and optional remote monitoring, so you know exactly how your run is progressing.

5. Sterile Sampling & Harvest

• Independent, sterilizable sampling valves help avoid contamination.
• Bottom harvest valves (Gemu/Bürkert) ensure zero-dead-zone discharge, with hot-sterilizable heads and optional auto-drain.

Fermenter Technical Parameters

PARAMETER	DESCRIPTION
Total volume	5 L, 10 L, 15 L, 20 L, 30 L, 50 L, 100 L, 200 L, 500 L, 1000 L, 5 KL, 50 KL, 200 KL, etc.
Working volume	30 % – 70 % of total volume
Tank	Jacketed SS316/SS304; tank pressure 0.3 MPa; jacket pressure 0.35 MPa; polished 0.4–0.6 µm; H:D ratio 1:2.2–2.5
Stirring system	Mechanical drive (top/bottom) or magnetic drive (top); 3 pcs height-adjustable sixblade paddles
Stir speed	50–1000 rpm (mechanical), 50–400 rpm (magnetic)
Sterilization	SIP (sterilize-in-place), auto‐controlled via program
Clean	CIP (clean-in-place) via spray ball
Gas control	Rotor meter; up to 1 vvm (standard)
Feeding	Automatic feeding via peristaltic pump
Anti-foam control	Auto antifoam via peristaltic pump
Temperature control	Auto-controlled; electric/water jacket range 5 °C–70 °C
pH control	2.00–12.00 ± 0.1, sensor: Hamilton or Mettler; peristaltic acid/base dosing
DO control	0–150 % ± 3 %, sensor: Hamilton or Mettler; coupling with agitation and aeration
Sampling	Manual sampling valve, sterilizable
Harvest	Zero-dead-zone bottom valve (Gemu/Bürkert, GER), sterilizable, manual or automatic
Lid lift system	30–500 L: hydraulic lift; 700–1000 L: manhole
Pressure	Auto-control via BD Germany pressure transmitter
Exhaust	Condenser + diaphragm or proportional control valve
Control parameters	10″ or 15″ touch screen; Siemens PLC; controls stirring, pH, DO, temp, feeding, gas, alarms; data logging; multi-language; remote/data output
Optional configuration	Remote control, mass flow meter, O₂/N₂/CO₂, 2 vvm gas, CO₂ exhaust analysis, level/turbidity/OD/ORP sensors, weight/live cell sensors, printing, Siemens HMI, double mechanical seal, 4-valve feeding group, auto bottom discharge, ASME/CSA certification, batch/seed modes

 

Applications

This versatile platform supports an array of biological processes:

• Animal vaccines:Avian influenza, PRRS, FMD, rabies, mink, DTP, diphtheria-(tetanus
• Human vaccines:diphtheria, tetanus, meningitis, pneumonia IV, hepatitis B
• Microbial cultures:bacteria, fungi, yeast, enzyme and antibiotic production included
• Bio-cell lines:Unicellular mammalian, such as CHO, HEK293, Vero, etc. cell lines (biologics, antibodies, viral vectors)
• Plant and algal cultures:secondary metabolites, recombinant proteins, biofuels
• Industrial enzymes and proteins:high purity production capability to be used in pharma or diagnostics products

This is the Swiss army bike of bioreactor systems, all certified and capable of being put in service with challenging R&D and pilot-scale manufacture.

Why Choose the Three‑Stage Multi‑Stage Fermenter 10L‑50L‑100L?

One Device, Unlimited Configurations

Effortlessly scale from gram to kilogram yield, without switching equipment.

Accuracy You Can Trust

Premium sensors, German valves, Siemens PLC in such a way to be reliable in terms of data and challenging regulatory compliance.

Maximize Productivity, Minimize Contamination

Zero-dead design, touchless, sterile valves and Integrated SIP/CIP is intended to minimize biohazard as well as down time.

Lab-Friendly, Operator-Loving

Easy to read controls, safe hydraulics, stainless-shipped contoured vessel-designed to save the operator fatigue.

Future-Proof Investment

Various modular add-ons, such as mass flow meters, live cell sensors, CO 2 exhaust monitoring, and remote control facilitate the changes in process requirements.

Who Should Consider This Fermenter?

The Three-stage multi-stage fermenter 10L-50L-100L is perfect for:

• Academic core labs scaling from flask to pilot
• Biotech firms developing vaccines, enzymes, antibodies
• Contract research and development/contract development and manufacturing operations that require a flexible capacity and control over a process
• Startups producing biologics, microbial products, or novel cell therapies

In short, when sterility, traceability, control, and of course, flexibility, need to be applied to the work, this system is tailored to your needs.

Take the Next Step

Do you want to revolutionize your R&D or scale-up? The Three-stage multi-stage fermenter 10L-50L-100L provides:

• Scalable capacity (10, 50, 100 L and beyond)
• Clean, precise design
• Sophisticated yet user-friendly automation
• Tailored configurations, validated components
• Global support & certifications

Let’s own your next breakthrough, whether it’s vaccine production, enzyme yield ramp, therapeutic cell processes, or anything else, right from bench to pilot.

Final Thoughts

Through its modular growth potential, stainless steel enhancements, fully automatic optimization of process through Siemens PLC, and a complete line of accessories and documentation- the Three-stage multi-stage fermenter 10L-50L-100L is the cream of the crop R&D and pilot-scale bioreactor platform. Figure: Ready to achieve better reproducibility, simplify scale‑up and raise reliability of various bioprocesses.

Is your laboratory prepared to go into the next generation of fermentation? Let’s talk.

FAQ’s

Q: Can I run only one stage (e.g., just the 50 L)?

A: Absolutely! Every stage works separately, you may mix several of them or run them alone, depending on your protocol.

Q: What sterilization cycles are supported?

A: Full Pressurized steam cleaning in SIP cover followed by cleaning of jacket and heads. CIP is done via built-in spray ball. Both are PLC-managed.

Q: What’s the maximum sustainable run time?

A: These systems can be maintained under multi-week continuous or fed-batch operation with data integrity via automatic logging using good quality sensors and control.

Q: Can I harvest cells without opening the vessel?

A: Yes. Our hygienic bottom valve, either manually or auto discharge, removes the opportunity of exposure or dead zones.

Q: Does it support GMP and regulatory records?

A: Yes, there are ASME, CSA, data export, alarms, printouts and validation procedures available to facilitate GMP.