2000L Multi-stage Industrial Bioreactor

Industrial Bioreactor Flexible Design: Work Independently or in Combination

It is a rare occasion that modern day fermentation is carried out in a single tank. That is why 2000L Multi-stage Industrial Bioreactor deals with:

• Multi-step installation: tanks can be used individually or linked together one to another
• Reliable, sterile transplantation pipelines between tanks
• Floor or bench based racks to suit your plant system
• Separate or centralized Siemens PLC control cabinets

Consider beginning your fermentation process on a seed fermenter followed by a smooth transition to larger and larger tanks all the way to production in an uncompromised way between sterility and efficiency.

Would you like to have multi-stage option in your process?

Industrial Bioreactor Technical Parameters

Parameter	Description
Total volume	5L,10L,15L,20L,30L,50L,100L,200L,500L,1000L, 5KL, 50KL, 200KL etc.
Working volume	30%~70%
Tank	Jacket tank, made by SS316 and SS304, tank design pressure 0.3Mpa, jacket design pressure 0.35Mpa, polishing 0.4-0.6um, ratio of diameter and height is 1:2.2-2.5
Stirring system	Mechanical drive on the top/bottom or Magnetic drive on the top, 3pcs height adjustable six-blade paddle Stir speed: 50~1000rpm/50-400rpm
Sterilization	Sterilize in place(SIP), auto control by program
Clean	Clean in place (CIP) by spray ball
Gas control	Control by rotor meter, air flow 1 vvm
Feeding	Automatically feeding by peristaltic pump
Anti-foam control	Automatically add antifoam by peristaltic pump
Temperature control	Auto-control,  electric heating range: water source add 5℃ to 70℃
PH control	PH 2.00-12.00±0.1, auto-control adding acid/base by peristaltic pump, pH sensor from Hamilton/Mettler, Switzerland.
Do control	0-150±3% displaying precision 0.1%, DO sensor from Hamilton/Mettler, Switzerland. DO coupling with agitation, oxygen.
Sampling	Sampling valve, can sterilization separately, manual
Harvest	Zero dead zone bottom valve(Gemu/Burket,Germany), can sterilization separately, manual/auto
Lid lift system	30L-500L have lid lift system, 700-1000L have human hole.
Pressure	Auto control, BD Germany pressure transmitter
Exhaust	Have condenser before exhaust, control by diagram valve / Proportional control valve.
Control parameters	10 or 15 inch touch screen , Siemens PLC with control parameters (stirring, pH, DO, temperature, feeding, alarm, gas etc.), data analysis, data record, data output, language(RU/EN/CN) and other customized functions
Optional configuration	Remote control, Mass flow meter(auto control gas), O2/N2/CO2, gas flow 2vvm, gas(CO2) exhaust analysis, Level sensor, Turbidity(OD) sensor, ORP sensor, Weight system, Live cell sensor(Biomass), Printing, Siemens touch screen, Double mechanical seal, 4-Valves-group for feeding, Bottom valve auto discharge, ASME certificate, CSA certificate, Batch culture, Seed transfer, etc.

Why Choose Stainless Steel for Industry-Scale Fermentation?

When you’re producing at 2000 liters or more, every weld, seal, and valve matters. It has SS316L and SS304 stainless steel constructed fermenter tank:

• Superior corrosion resistance to withstand acids and bases
• Polished internal surfaces (0.4–0.6μm) to reduce contamination risk
• No dead zones with rounded corners and a customized practice of welding
• High design pressure for processes demanding temperature swings

Do your existing tanks have a design that is designed to last 100 days a day?

Intelligent Mixing: Stirring & Gas Flow in Harmony

• Each batch has unique shear, oxygen, and mixing needs.
• As an industry fermenter, select the Multi-stage stainless steel 2000L.
• Top or bottom mechanical drives
• Magnetic drive option (top) for reduced contamination risk
• 3 heights‑adjustable six‑blade paddles
• Wide stir speed range (50–1000rpm)

Combined with gas flow control (rotor meter, 1 vvm standard; mass flow optional), you get fine-tuned oxygen transfer for cell cultures, yeast, or bacteria.

Temperature & pH Under Precise Control

Correct control is not a luxury; it is the grant to reproducibility.

✅ Auto‑controlled electric heating

✅ Wide range: +5℃ to 70℃

✅ Siemens PLC‑driven pH control: pH 2.00–12.00 ±0.1

✅ Sensors from Hamilton/Mettler for reliability

Stable temperature + pH = consistent product quality.

Sterility by Design: SIP & CIP

Contamination can ruin days, or weeks, of work.

With this system:

• SIP:Sterilization in place, automated
• CIP:Cleaning in place, spray ball ensures full coverage
• Sterile sampling & harvest valves:can be sterilized separately
• Sterile filtration:2μm filters prevent backflow

How much time (and risk) could you save with true SIP & CIP?

Siemens Automation: Control, Monitor, Optimize

Run the fermenter through:

• 10” or 15” color touchscreen
• Siemens S7-200 PLC
• On‑screen trends, alarms, and data export
• Multilingual menus (RU/EN/CN)
• Optional remote monitoring & batch recording

Upgrades? Include live cell sensors, ORP, turbidity, exhaust CO 2 analysis and so on.

Typical Applications: Animal & Human Vaccines, Bio‑production

Imagine the scale:

• Animal vaccines:PRRS, rabies, mink, DTP, tetanus
• Human vaccines:hepatitis B, pneumonia, meningitis
• Biotech:enzymes, proteins, antibiotics, probiotics
• Culture:bacteria, yeast, fungi, CHO / 293 Cells, algae, plant cells

The 2000L Multi-stage Industrial Bioreactor is the interpreter between pilot and production.

A True Multi‑stage System: Why It Matters

With multi-stage setups, you can:

• Propagate seed cultures to a grow condition in 1/10th of production scale tanks → scale up to production
• Reduce contamination risk (separate sterilization)
• Scale process while keeping control over each step
• Floor-standing or bench-mounted; separate or centralized control.

Will dividing your process in stages increase yield and cut failure rate?

Built to Last, Designed for Safety

• German BD pressure transmitters for precise control
• Large viewing glass & 12V safe lighting
• Sampling / inoculation / feeding ports + spares
• Optimized flow design = uniform temperature & nutrient mixing
• Polished welds: strong, clean, and hygienic

What Comes Standard?

✅ Fully jacketed 2000L SS316L/304 tank

✅ Mechanical / magnetic stirring system

✅ Siemens PLC + touchscreen

✅ Hamilton/Mettler sensors (pH & DO)

✅ CIP & SIP functions

✅ Peristaltic pumps for feed & antifoam

✅ Rotor meter for gas flow

✅ Sampling & harvest valves

✅ Exhaust condenser & valves

✅ Documentation & training

Customization: Because Every Process is Different

Add:

• Mass flow meters for automated gas blending
• O₂, N₂, CO₂ control lines
• Remote monitoring & batch data export
• Auto discharge valves & bottom valves
• ASME/CSA certification
• Batch culture & seed transfer software
• ORP, turbidity, biomass sensors

Let us design your perfect setup.

Why choose BaiLun Bio?

✅ 10+ years serving global biotech & pharma

✅ Siemens automation as standard

✅ CE / ISO / ASME certified designs

✅ Stainless steel built for longevity

✅ Support & spare parts worldwide

✅ Flexible to scale with your needs

From Lab Bench to Production

Start with 5–10L, then move to 2000L, all with consistent:

• Automation
• Control logic
• Sensor technology
• Same platform, easier scale-up, faster time to market.

Imagine the Possibilities

What if you could:

• Reduce contamination events by 80%?
• Cut cleaning time in half?
• Double yield consistency batch-to-batch?

That’s the value behind engineering, not just volume.

Ready to redefine your process?

Discover 2000L Multi-stage Industrial Bioreactor now.

• Visit BaiLun Bio
• Request a quote
• Ask technical questions
• Discuss your process needs

The future of controlled, large‑scale fermentation starts here.