Four-stage stainless-steel fermenter 20L-200L-1000L-200L (Built to precision and designed with power)

Do you want to find a fermentation system that combines scaling, trustworthiness, and overall control? 四级不锈钢发酵罐20L-200L-1000L-200L is not only a laboratory equipment, but an essential part of bioprocess innovative work.

Whether treating your microbial fermentation, growing mammalian cultures, or up scaling vaccine manufacture, this four stage apparatus offers smooth blending of the high technology level of controls with the enduring strength and efficiency of stainless steel that only stainless steel can provide.

But what makes this system truly exceptional? Let’s explore!

Why Choose a Four-Stage Stainless-steel Fermenter 20L-200L-1000L-200L ?

In modern bioprocessing, flexibility and process control are everything. The 四级不锈钢发酵罐20L-200L-1000L-200L provides researchers and manufacturers with possibility to:

• Run multiple fermentation stages in sequence or parallel
• Scale from development to production in a single system
• Maintain sterile, controlled environments across all stages
• Automate vital processes such as feeding process, anti-foam, PH adjustment and logging functions

Are you looking to have a solution to support your fermentation strategy? Whether it’s batch, fed-batch, or continuous mode, this system handles it all.

Compact. Automated. Intelligent.

This high performance fermenter line BLBIO-20SJA-200SJA-1000SJA-200SJA was developed to work and to be user friendly. It includes an efficient compact format with all elements in a unified stainless-steel installation and the high automation implies less direct control and greater capacity to repeat findings.

Each fermenter in the four-stage setup includes:

• Automatic SIP (Sterilization in Place) and CIP (Clean in Place)
• Peristaltic pumps for acid, base, antifoam, and nutrient addition
• High-precision sensors for pH, DO, temperature, and pressure
• Siemens PLC + Touchscreen interface with complete control visibility

And what would happen, once you can control all the processes: acids addition, cell harvesting, and so on, only by a couple of taps? With this system, you can.

Who is This Four-Stage Stainless-steel Fermenter 20L-200L-1000L-200L for?

• Biotech companies scaling from lab to pilot plant
• Pharmaceutical R&D teams exploring vaccines, antibiotics, proteins
• Government Research labs and academic labs with complicated mammalian cell or microbial cultures
• Researchers of fermentation required several vessels, which have independent workflows or simply connected workflows

The four stage arrangement also enables an easy move between the seed preparation, seed growth, production and harvesting. You can run each vessel separately or in unison.

Deep Dive into the Four-Stage Stainless Steel Fermenter

The distinctive feature of the most of demanding fermentation needs is that all the four vessels, namely 20L, 200L, 1000L, and 200L are designed.

1. Smart Stirring System

• Depending on your process requirements, each tank features:
• Top mechanical stirring or magnetic bottom drive
• Adjustable six-blade impellers
• Precision motor control (50–1000 rpm for mechanical; 50–400 rpm for magnetic)

Mixing mammalian cells is far more delicate than mixing high shear microbial broths, but this system offers the consistency required of a viable and repeatable growth.

2. Advanced Process Monitoring

A Siemens 10″ or 15″ color touchscreen displays:

• All sensor readings in real-time
• Set point adjustments
• Alarm status
• Trends & historical data

The four fermenters can be linked to a common or specific controller and they can be also linked to external computers to be able to export the data as well as to provide a remote control.

3. Peristaltic Precision

Four inbuilt peristaltic pumps in each control box make it possible:

• Nutrient media addition
• Base/acid control for pH balancing
• Antifoam dosing
• Sampling or product addition

Do you have the capability to automate the whole of your run give inoculation and harvest? With this system, yes.

技术规格

范围	Description
总容积	5 L, 10 L, 15 L, 20 L, 30 L, 50 L, 100 L, 200 L, 500 L, 1000 L, 5KL, 50KL, 200KL, etc.
工作量	30–70 % of total volume
Tank construction	Jacketed tanks, SS316/SS304; tank pressure 0.3 MPa; jacket 0.35 MPa; inner polish 0.4–0.6 µm; height:diameter ratio 1:2.2–2.5
搅拌系统	Top/bottom mechanical or top magnetic drive; 3 height-adjustable six-blade paddles
Stirring speed	50–1000 rpm (mechanical); 50–400 rpm (magnetic)
消毒	Sterilization in place (SIP), fully automated
打扫	Clean in place (CIP) with built-in spray ball
气体控制	Rotameter control; standard 1 vvm airflow
喂养	Peristaltic pump-based automatic feeding
Anti-foam	Automatically added by peristaltic pump
温度控制	Auto control; heating range: water source +5 °C to 70 °C
pH control	2.00–12.00 ±0.1; acid/base peristaltic dosing; Hamilton/Mettler pH sensor
溶解氧控制	0–150 % ±3 %; DO sensors from Hamilton/Mettler; coupled with agitation and gas input
采样	Manual, with separately sterilizable valve
Harvesting	Bottom zero-dead-zone valves (Gemu/Bürkert Germany), manual/auto options
Lid system	30–500 L: hydraulic lid lift; 700–1000 L: human access port
Pressure control	Auto-controlled using BD Germany pressure transmitter
Exhaust control	Condenser + diaphragm valve or proportional control valve
Control interface	Siemens PLC with 10″/15″ touch screen; multilingual UI (RU/EN/CN); supports alarms, gas control, pH, DO, temperature, feeding, and logging
Optional add-ons	Remote control, mass flow meters, O₂/N₂/CO₂ integration (2 vvm), CO₂ exhaust analyzer, turbidity sensor, ORP sensor, biomass sensor, auto-discharge, ASME/CSA

Applications in Real-World Bioprocessing

Not only does this four-stage system not apply to just industry, it enables many industries among them:

Human and Animal Vaccines

• Hepatitis B, pneumonia, diphtheria, tetanus
• Avian flu, rabies, PRRS, foot-and-mouth disease

Microbial and Fungal Fermentation

• Bacteria (E. coli, B. subtilis)
• Yeast (Pichia, Saccharomyces)
• Fungi (Aspergillus, Trichoderma)

Mammalian & Plant Cell Culture

• CHO, HEK293, Vero lines
• Recombinant protein or antibody production

Bio-products

• Enzymes, probiotics, amino acids
• Nutritional supplements, food additives
• Antibiotics and organic acids

Want the ability to scale to flask to production in a single system? This fermenter makes it not only possible, but effortless.

What Makes This Fermenter Unique?

• Four interconnected stages (20L to 1000L) allow complete bioprocess chains in one solution
• Modular controls and vessel operation, independent or simultaneous
• Sterile transfer lines, auto harvest, and automated feedback-based control
• Top-tier components from Germany and Switzerland
• Multi-language, remote-ready interface for international teams

这 四级不锈钢发酵罐20L-200L-1000L-200L isn’t just a fermenter, it’s a fully integrated bioreactor suite.

Ready to Take Control of Your Fermentation Process?

四级不锈钢发酵罐20L-200L-1000L-200L is not an ordinary laboratory piece of equipment. It is an all-in-one powerhouse that grows with you, evolves to your needs and makes even the most complex protocols of fermentation simple.

Why settle for less when you can have:

• Multi-stage control
• Accurate and repeatable results
• Automated operation
• Scalability from R&D to production

So, whether you’re developing the next life-saving vaccine or scaling up an innovative food biotech product, this system delivers the flexibility, performance, and data control you need.

Don’t hesitate to reach us now to get to know more or book a virtual demo or even a quote.

Frequently Asked Questions (FAQ’s)

Q: Is it possible to use only one or two fermenters only?

A: Yes, you can use any single vessel away from the other depending on your scale or what step you are doing in the process.

Q: How does SIP/CIP work across multiple tanks?

A: both vessels incorporate SIP / CIP stand alone and may be sterilized and cleaned simultaneously or sequentially in programmed cycle.

Q: Is this fermenter suitable for GMP-certified production?

A: Absolutely. The system has the ASME/CSA certification and the Siemens traceability making it comply with GMP and other international quality requirements.

Q: What kind of data output is available?

A: Logs, graphical and control data can be exported directly out of the PLC interface or by linked software systems to QC or regulatory records.

Q: Can I automate gas mix ratios?

A: Yes! Same with optional mass flow meters and CO 2 analysis sensors, only having control to automate and tune O 2 /N 2 /CO 2 blends to precision culture conditions.