Unlock Advanced Bioprocessing with the Two‑stage multi‑stage fermenter 10L‑100L

Have you ever been in a situation that you wished you could build up your microbial or cell culture process effectively and without having to buy any separate systems? Or perhaps you’re aiming to run parallel experiments while maintaining utmost control and automation? The 两级多级发酵罐10L-100L manufactured by BaiLun Bio is a groundbreaking product solution- it combines the advantages of two-staged fermentation, such flexible volume and strong functions that fermentation is unified and simplified.

Whether you’re performing batch cultures, fed-batch processes, continuous fermentation, or scale-up experiments, this system is tailored to empower R&D teams, biotech innovators, and academic researchers. Let’s explore how this all-in-one platform could transform your lab’s fermentation capabilities.

What Makes the Two‑Stage Multi‑Stage Fermenter 10L‑100L Unique?

• Two-stage design:This is an arrangement in which there are two jacketed fermenters that may either run under the same or different conditions resulting in smooth parallel or cascading fermentation.
• Volume scalability:As the volumes range between 10 L and 100 L, the system works at different project sizes – it is flexible to grow even bigger.
• Sophisticated automation:Sterilization-in-place (SIP), clean-in-place (CIP), feeding, foaming, pH/DO/temperature control, fully programmatic.
• Intelligent design:An obvious intelligent design is included in jacketed pressure vessels, sight glass, piping to an upward direction (steam pipe), safety valves, and stainless steel materials that will prevent sterility, enhance efficiency, and durability.

Are you prepared to enter continuous production, sophisticated screening or pilot-scale all at the same intuitive platform?

Who Benefits from This System?

• Biotech R&D groups:process direct comparison of strains, feeding optimization or metabolic studies.
• Vaccine and therapeutics manufacturers:Grow CHO or HEK cell cultures to high accuracy under controlled conditions.
• Industrial enzyme and biofuel developers: Testtwo-stage processes efficiently.
• Academic labs:Teach students with an industrial-grade two-tank setup.
• Pilot facilities:Test processes, and guidelines on a scale appropriate to scale-up.

Just suppose, you have two fermenters in a cabinet–but two capable of different work. That’s the flexibility you get with this platform.

Technical Specifications Overview

PARAMETER	DESCRIPTION
Total volume options	5 L, 10 L, 15 L, 20 L, 30 L, 50 L, 100 L, 200 L, 500 L, 1000 L, 5 KL, 50 KL, 200 KL
工作量	30––70 %
Tanks	Dual jacketed fermenters (SS304/SS316L), pressure-rated to 0.3 MPa with 0.35 MPa jackets, polished 0.4–0.6 μm, diameter:height = 1:2.2–2.5
Stirring systems	Mechanical drive (top or bottom) or magnetic drive (top); 3 adjustable six-blade paddles, 1 foam breaker
Stir speed	50–1000 rpm (mechanical) / 50–400 rpm (magnetic)
消毒	SIP (sterilize-in-place), fully automated
打扫	CIP (clean-in-place) via spray ball
气体控制	Rotameter-controlled, 1 vvm airflow
喂养	Automatic peristaltic feeding (media, acid, base, antifoam)
消泡控制	Automatic antifoamer addition
温度控制	Jacket heating and water-source, programmable from 5 °C to 70 °C
pH control	Range 2.00–12.00 ±0.1; pH sensors from Hamilton/Mettler; acid/base auto dosing
溶解氧控制	0–150 % ±3% with 0.1% resolution; DO sensors from Hamilton/Mettler; dynamic coupling with agitation and oxygen input
采样	Separate sterilizable sampling valves
Harvest/Dismantling	Zero-dead-volume bottom valves (Gemu/Bürkert); standalone sterilization; manual/automatic harvest
Lid system	Lid-lift for 30–500 L tanks; manhole for 700–1000 L
Pressure monitoring	Auto pressure control via BD Germany transmitter
Exhaust system	Pre-exhaust condenser + PID-controlled diaphragm/proportional exhaust valve
Control interface	Siemens S7-200 PLC + 10/15″ touch screen; multilingual (EN/RU/CN); control over all fermentation parameters with data recording and export
Optional modules	Remote control, mass flow meters, O₂/N₂/CO₂ (2 vvm gas), gas CO₂ exhaust analysis, level/OD/ORP sensors, weighing, live BIOMASS sensors, printing, double mechanical seals, harvest automation, ASME/CSA certification, staging, seed transfer piping, batch mode support

Flexible Two-Tank Operation

Parallel or Cascade Fermentation

• Parallel mode:Do the two independent fermentations: this is best used when you want replicates, comparisons, or even when conditions need to be conducted side by side.
• Cascade (two-stage) mode:Stage one cultivates biomass, stage two focuses on product formation. Both stages can be independently optimized.

Continuous Fermentation Capability

Conduct both stage one and stage two as continuous (Unless stage two is run as batch), which results in a semi-continuous bioprocess. Experimental design just got easier and more powerful.

CIP & SIP: Maintenance-Free Bioprocess

• At high temperatures sterilizing the tanks and piping is possible with SIP feature eliminating manual disassembly.
• CIP employs the spray ball technology to guarantee cleanliness of the vessels at completion of each cycle.

This will save several hours of downtimes and cross-contamination risk.

Advanced Control & Operation

• Siemens PLC with Intuitive Touchscreen
• Watch the real-timing parameters: the rate of stirring, temperature, pH, DO, gas flow, and feed schedule are often depicted in a graphical form.
• Store, export and analyze the data by CSV output and have all traceability.
• Alarm functions notify key thresholds (temperature, pH shifts, pressure).
• Accessible in multiple languages (EN/RU/CN), ideal for international teams.

Independent or Shared Control

Choose flexibility:

• Shared control simplifies calibration and duplicates conditions.
• Experimental diversity among tanks is possible, which is simple to change modes with the usage of the UI.

Safety and Sterility First

• Zero-dead valves ensure full transfer and prevent buildup.
• Media loss is avoided and exhaust lines are sterile due to the use of condenser and sterile filters.
• Pressure transmitter ensures that the vessel remains safe whilst hygienic design prevents cross contamination.

实际应用

R&D Parameter Screening

Compare two or more strains, feeding programs or temperature regimes, at the same time in two fermenters to reduce testing times by 50 per cent.

Two-Stage Product Development

Stage one: biomass production, stage two: product induction-suitable with enzymes, secondary metabolites and vaccine antigens.

Continuous Microbial Processes

Fed-batch with continuous (stage one) and Triggered Batch (stage two) are to be used together, in order to optimize fermentation.

哺乳动物细胞培养

Create CHO, HEK or insect cell cultures in the presence of controlled conditions with gentle mixing and temperature stability ideal in production of therapeutic proteins.

Academic & Pilot-Scale Testing

Learn, test and train your students in industrial level fermentation or certify protocols to scale up with this easily adjustable TWO vessel system.

How It Improves Your Workflow?

• Speed:View screen variables simultaneously in two machines instead of manually in series
• 一致性：Shared hardware & calibration reduce batch variability
• 可扩展性：scale up to 1000 L and higher with options ranging between 10-100 L
• 效率：Fully automated cleaning, sterilization, and sampling
• Safety:There is zero-dead zones, hygienic valves, and sterile exhaust systems that promote integrity
• Data Empowerment:Plot and export data directly out of the controller- no manual archives

Next Steps: Configure Your Setup

Check the Two-stage Multi-stage fermenter 10L-100L product webpage at the Bai ln Bio website.

• Choose your tank volumes (e.g., 10 + 10 L, or 50 + 50 L).
• Select your stirring configuration (mechanical or magnetic).
• Add sensor options, OD, biomass, ORP.
• Available accessories: remote control, gas mixing, feed valve packs, automated discharge etc.
• Download the data sheet or schedule a demo.

结论

With screen-to-scale-to pilot, gains of speed, flexibility and integrity of data, the 两级多级发酵罐10L-100L equips labs with scale span of 10L100L. Run dual experiments in parallel, keep downtime to the minimum and have a strict control over your process, all in a versatile, scalable system.

Ready to streamline fermentation, accelerate research, and simplify scale? 联系我们 to ask how this game-changing platform could work for your projects, now and in months to come.

Frequently Asked Questions (FAQs)

Q1: Can both fermenters work independently?

Yes, stirring, temperature, pH, DO, gas and feed have been independently controlled in each tank. Select shared or independent control via touchscreen.

Q2: How is sterilization performed?

The system forms SIP- connect to steam, start sterilizing through PLC and the system does the heating, hold time and cool down.

Q3: Are data export and integration options available?

Yes, of course: the data may be exported by USB or Ethernet as CSV to LIMS or analytics systems. Option for remote network control is also available.

Q4: Is it easy to harvest and discharge cultures?

Yes, all the harvest ports carry the advantage of sterile, hygienic manual or automatic discharge by virtue of the zero-dead- valve.

Q5: What certifications are available?

Regulated environments can be placed under optional ASME compliance or CSA compliance. Complete customization is achieved through extra modules such as double seals, exhaust analysis, sensor upgrades etc.